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PDBsum entry 5e34
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Viral protein
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PDB id
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5e34
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References listed in PDB file
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Key reference
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Title
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Structural basis for a switch in receptor binding specificity of two h5n1 hemagglutinin mutants.
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Authors
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X.Zhu,
K.Viswanathan,
R.Raman,
W.Yu,
R.Sasisekharan,
I.A.Wilson.
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Ref.
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Cell Rep, 2015,
13,
1683-1691.
[DOI no: ]
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PubMed id
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Abstract
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Avian H5N1 influenza viruses continue to spread in wild birds and domestic
poultry with sporadic infection in humans. Receptor binding specificity changes
are a prerequisite for H5N1 viruses and other zoonotic viruses to be transmitted
among humans. Previous reported hemagglutinin (HA) mutants from
ferret-transmissible H5N1 viruses of A/Vietnam/1203/2004 and A/Indonesia/5/2005
showed slightly increased, but still very weak, binding to human receptors. From
mutagenesis and glycan array studies, we previously identified two H5N1 HA
mutants that could more effectively switch receptor specificity to human-like
α2-6-linked sialosides with avidity comparable to wild-type H5 HA binding to
avian-like α2-3-linked sialosides. Here, crystal structures of these two H5 HA
mutants free and in complex with human and avian glycan receptor analogs reveal
the structural basis for their preferential binding to human receptors. These
findings suggest continuous surveillance should be maintained to monitor and
assess human-to-human transmission potential of H5N1 viruses.
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