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PDBsum entry 5dwc
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References listed in PDB file
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Key reference
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Title
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Restriction endonuclease agei is a monomer which dimerizes to cleave DNA.
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Authors
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G.Tamulaitiene,
V.Jovaisaite,
G.Tamulaitis,
I.Songailiene,
E.Manakova,
M.Zaremba,
S.Grazulis,
S.Y.Xu,
V.Siksnys.
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Ref.
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Nucleic Acids Res, 2017,
45,
3547-3558.
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PubMed id
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Abstract
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Although all Type II restriction endonucleases catalyze phosphodiester bond
hydrolysis within or close to their DNA target sites, they form different
oligomeric assemblies ranging from monomers, dimers, tetramers to higher order
oligomers to generate a double strand break in DNA. Type IIP restriction
endonuclease AgeI recognizes a palindromic sequence 5΄-A/CCGGT-3΄ and cuts it
('/' denotes the cleavage site) producing staggered DNA ends. Here, we present
crystal structures of AgeI in apo and DNA-bound forms. The structure of AgeI is
similar to the restriction enzymes that share in their target sites a conserved
CCGG tetranucleotide and a cleavage pattern. Structure analysis and biochemical
data indicate, that AgeI is a monomer in the apo-form both in the crystal and in
solution, however, it binds and cleaves the palindromic target site as a dimer.
DNA cleavage mechanism of AgeI is novel among Type IIP restriction endonucleases.
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