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PDBsum entry 5cbh
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Transport protein
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PDB id
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5cbh
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References listed in PDB file
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Key reference
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Title
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Structural and functional characterization of a calcium-Activated cation channel from tsukamurella paurometabola.
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Authors
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B.Dhakshnamoorthy,
A.Rohaim,
H.Rui,
L.Blachowicz,
B.Roux.
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Ref.
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Nat Commun, 2016,
7,
12753.
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PubMed id
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Abstract
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The selectivity filter is an essential functional element of
K+channels that is highly conserved both in terms of its primary
sequence and its three-dimensional structure. Here, we investigate the
properties of an ion channel from the Gram-positive bacterium Tsukamurella
paurometabola with a selectivity filter formed by an uncommon proline-rich
sequence. Electrophysiological recordings show that it is a non-selective cation
channel and that its activity depends on Ca2+concentration. In the
crystal structure, the selectivity filter adopts a novel conformation with
Ca2+ions bound within the filter near the pore helix where they are
coordinated by backbone oxygen atoms, a recurrent motif found in multiple
proteins. The binding of Ca2+ion in the selectivity filter controls
the widening of the pore as shown in crystal structures and in molecular
dynamics simulations. The structural, functional and computational data provide
a characterization of this calcium-gated cationic channel.
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