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PDBsum entry 5c8q
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Sugar binding protein
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PDB id
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5c8q
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References listed in PDB file
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Key reference
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Title
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Structural insight into fungal cell wall recognition by a cvnh protein with a single lysm domain.
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Authors
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L.M.Koharudin,
K.T.Debiec,
A.M.Gronenborn.
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Ref.
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Structure, 2015,
23,
2143-2154.
[DOI no: ]
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PubMed id
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Abstract
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MGG_03307 is a lectin isolated from Magnaporte oryzae, a fungus that causes
devastating rice blast disease. Its function is associated with protecting
M. oryzae from the host immune response in plants. To provide the structural
basis of how MGG_03307 protects the fungus, crystal structures of its CVNH-LysM
module were determined in the absence and presence of GlcNAc-containing cell
wall chitin constituents, which can act as pathogen-associated molecular
patterns. Our structures revealed that glycan binding is accompanied by a
notable conformational change in the LysM domain and that GlcNAc3 and GlcNAc4
are accommodated similarly. GlcNAc5 and GlcNAc6 interact with the LysM domain in
multiple conformations, as evidenced by solution nuclear magnetic resonance
studies. No dimerization of MoCVNH3 via its LysM domain was observed upon
binding to GlcNAc6, unlike in multiple LysM domain-containing proteins.
Importantly, we define a specific consensus binding mode for the recognition of
GlcNAc oligomers by single LysM domains.
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