UniProt functional annotation for P36544



	UniProt functional annotation for P36544

UniProt code: P36544.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.

Subunit: Homopentamer (By similarity). Interacts with RIC3; which is required for proper folding and assembly (PubMed:15504725, PubMed:16120769). Interacts with LYPD6 (PubMed:27344019). Interacts with the alpha-conotoxin RgIA (By similarity). Interacts with alpha- conotoxins ImI and ImII (PubMed:15609996). Interacts with CANX (PubMed:32783947). {ECO:0000250|UniProtKB:P54131, ECO:0000250|UniProtKB:Q05941, ECO:0000269|PubMed:15504725, ECO:0000269|PubMed:15609996, ECO:0000269|PubMed:16120769, ECO:0000269|PubMed:27344019, ECO:0000269|PubMed:32783947}.

Subcellular location: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein {ECO:0000255}. Cell membrane {ECO:0000269|PubMed:27789755}; Multi-pass membrane protein {ECO:0000255}. Note=TMEM35A/NACHO promotes its trafficking to the cell membrane (PubMed:27789755). RIC3 promotes its trafficking to the cell membrane (By similarity). {ECO:0000250|UniProtKB:Q05941, ECO:0000269|PubMed:27789755}.

Ptm: Glycosylations at Asn-46, Asn-90 and Asn-133 are essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to the cell membrane. {ECO:0000250|UniProtKB:P49582}.

Mass spectrometry: Mass=54157.68; Method=MALDI; Evidence={ECO:0000269|PubMed:11840567};

Miscellaneous: [Isoform 3]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. {ECO:0000305}.

Similarity: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub- subfamily. {ECO:0000305}.

Sequence caution: Sequence=AAH37571.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.


Subunit:		Homopentamer (By similarity). Interacts with RIC3; which is required for proper folding and assembly (PubMed:15504725, PubMed:16120769). Interacts with LYPD6 (PubMed:27344019). Interacts with the alpha-conotoxin RgIA (By similarity). Interacts with alpha- conotoxins ImI and ImII (PubMed:15609996). Interacts with CANX (PubMed:32783947). {ECO:0000250\|UniProtKB:P54131, ECO:0000250\|UniProtKB:Q05941, ECO:0000269\|PubMed:15504725, ECO:0000269\|PubMed:15609996, ECO:0000269\|PubMed:16120769, ECO:0000269\|PubMed:27344019, ECO:0000269\|PubMed:32783947}.
Subcellular location:		Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein {ECO:0000255}. Cell membrane {ECO:0000269\|PubMed:27789755}; Multi-pass membrane protein {ECO:0000255}. Note=TMEM35A/NACHO promotes its trafficking to the cell membrane (PubMed:27789755). RIC3 promotes its trafficking to the cell membrane (By similarity). {ECO:0000250\|UniProtKB:Q05941, ECO:0000269\|PubMed:27789755}.
Ptm:		Glycosylations at Asn-46, Asn-90 and Asn-133 are essential for TMEM35A/NACHO-mediated proper subunit assembly and trafficking to the cell membrane. {ECO:0000250\|UniProtKB:P49582}.
Mass spectrometry:		Mass=54157.68; Method=MALDI; Evidence={ECO:0000269\|PubMed:11840567};
Miscellaneous:		[Isoform 3]: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay. {ECO:0000305}.
Similarity:		Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub- subfamily. {ECO:0000305}.
Sequence caution:		Sequence=AAH37571.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305};