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PDBsum entry 5a6f
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References listed in PDB file
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Key reference
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Title
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Cryo-Electron microscopy structure of the slo2.2 na(+)-Activated k(+) channel.
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Authors
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R.K.Hite,
P.Yuan,
Z.Li,
Y.Hsuing,
T.Walz,
R.Mackinnon.
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Ref.
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Nature, 2015,
527,
198-203.
[DOI no: ]
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PubMed id
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Abstract
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Na(+)-activated K(+) channels are members of the Slo family of large conductance
K(+) channels that are widely expressed in the brain, where their opening
regulates neuronal excitability. These channels fulfil a number of biological
roles and have intriguing biophysical properties, including conductance levels
that are ten times those of most other K(+) channels and gating sensitivity to
intracellular Na(+). Here we present the structure of a complete Na(+)-activated
K(+) channel, chicken Slo2.2, in the Na(+)-free state, determined by
cryo-electron microscopy at a nominal resolution of 4.5 ångströms. The channel
is composed of a large cytoplasmic gating ring, in which resides the
Na(+)-binding site and a transmembrane domain that closely resembles
voltage-gated K(+) channels. In the structure, the cytoplasmic domain adopts a
closed conformation and the ion conduction pore is also closed. The structure
reveals features that can explain the unusually high conductance of Slo channels
and how contraction of the cytoplasmic gating ring closes the pore.
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