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UniProt functional annotation for P02920 | ||
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| UniProt code: P02920. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system). Can transport lactose, melibiose, lactulose or the analog methyl-1-thio-beta,D- galactopyranoside (TMG), but not sucrose or fructose (PubMed:1848449, PubMed:18177889, PubMed:22106930). The substrate specificity is directed toward the galactopyranosyl moiety of the substrate (PubMed:22106930). {ECO:0000269|PubMed:18177889, ECO:0000269|PubMed:1848449, ECO:0000269|PubMed:22106930}. |
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| Biophysicochemical properties: | |
Kinetic parameters: KM=0.62 mM for lactose {ECO:0000269|PubMed:18177889}; KM=0.24 mM for melibiose {ECO:0000269|PubMed:18177889}; KM=0.24 mM for lactulose {ECO:0000269|PubMed:22106930}; KM=0.54 mM for TMG {ECO:0000269|PubMed:18177889}; Vmax=191 nmol/min/mg enzyme with lactose as substrate {ECO:0000269|PubMed:18177889}; Vmax=105 nmol/min/mg enzyme with melibiose as substrate {ECO:0000269|PubMed:18177889}; Vmax=49 nmol/min/mg enzyme with lactulose as substrate {ECO:0000269|PubMed:22106930}; Vmax=180 nmol/min/mg enzyme with TMG as substrate {ECO:0000269|PubMed:18177889}; |
| Subunit: | |
Monomer. |
| Subcellular location: | |
Cell inner membrane {ECO:0000269|PubMed:2164211}; Multi-pass membrane protein {ECO:0000269|PubMed:2164211}. |
| Mass spectrometry: | |
Mass=47357; Method=Electrospray; Evidence={ECO:0000269|PubMed:10485888}; |
| Similarity: | |
Belongs to the major facilitator superfamily. Oligosaccharide:H(+) symporter (OHS) (TC 2.A.1.5) family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.