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UniProt functional annotation for Q44238 | ||
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| UniProt code: Q44238. |
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| Organism: | |
Alteromonas sp. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Alteromonas. |
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| Function: | |
Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position and a nonpolar amino acid at the N-terminal position. Also catalyzes the hydrolysis of toxic organophosphorus cholinesterase-inhibiting compounds including insecticide paraoxon and nerve gases such as diisopropylfluorophosphate (DFP), O-isopropyl methylphosphonofluoridate (sarin), O-pinacolyl methylphosphonofluoridate (soman), and O-cyclohexyl methylphosphonofluoridate. {ECO:0000269|PubMed:10866401, ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861, ECO:0000269|PubMed:9079288}. |
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| Catalytic activity: | |
Reaction=Hydrolysis of Xaa-|-Pro dipeptides. Also acts on aminoacyl- hydroxyproline analogs. No action on Pro-|-Pro.; EC=3.4.13.9; |
| Catalytic activity: | |
Reaction=diisopropyl fluorophosphate + H2O = diisopropyl phosphate + fluoride + 2 H(+); Xref=Rhea:RHEA:24100, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17051, ChEBI:CHEBI:17941, ChEBI:CHEBI:57896; EC=3.1.8.2; |
| Catalytic activity: | |
Reaction=An aryl dialkyl phosphate + H(2)O = dialkyl phosphate + an aryl alcohol.; EC=3.1.8.1; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:8633861}; Note=Binds 2 manganese ions per monomer. {ECO:0000269|PubMed:8633861}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=2.99 mM for diisopropylfluorophosphate {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; KM=1.57 mM for O-isopropyl methylphosphonofluoridate {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; KM=2.48 mM for O-pinacolyl methylphosphonofluoridate {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; KM=0.63 mM for O-cyclohexyl methylphosphonofluoridate {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; KM=1.27 mM for a chromogenic soman analog {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; Vmax=230 umol/min/mg enzyme with diisopropylfluorophosphate as substrate {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; Vmax=442 umol/min/mg enzyme with O-isopropyl methylphosphonofluoridate as substrate {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; Vmax=151 umol/min/mg enzyme with O-pinacolyl methylphosphonofluoridate as substrate {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; Vmax=652 umol/min/mg enzyme with O-cyclohexyl methylphosphonofluoridate as substrate {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; Vmax=52 umol/min/mg enzyme with a chromogenic soman analog as substrate {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; pH dependence: Optimum pH is 8.5 with DFP as substrate. {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:2001997, ECO:0000269|PubMed:8633861}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:2001997}. |
| Biotechnology: | |
Can be used for the catalytic detoxification of some nerve agents neurotoxins. |
| Miscellaneous: | |
Has a stereoselective preference for isomers with R- configuration at the phosphorous center of sarin and soman, and with S- configuration in phosphotriesters. |
| Similarity: | |
Belongs to the peptidase M24B family. Bacterial-type prolidase subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.