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PDBsum entry 4zto
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Immune system
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PDB id
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4zto
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Contents |
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216 a.a.
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221 a.a.
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11 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural analysis of a novel rabbit monoclonal antibody r53 targeting an epitope in HIV-1 gp120 c4 region critical for receptor and co-Receptor binding.
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Authors
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R.Pan,
Y.Chen,
M.Vaine,
G.Hu,
S.Wang,
S.Lu,
X.P.Kong.
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Ref.
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Emerg Microbes Infect, 2015,
4,
e44.
[DOI no: ]
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PubMed id
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Abstract
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The fourth conserved region (C4) in the HIV-1 envelope glycoprotein (Env) gp120
is a structural element that is important for its function, as it binds to both
the receptor CD4 and the co-receptor CCR5/CXCR4. It has long been known that
this region is highly immunogenic and that it harbors B-cell as well as T-cell
epitopes. It is the target of a number of antibodies in animal studies, which
are called CD4-blockers. However, the mechanism by which the virus shields
itself from such antibody responses is not known. Here, we determined the
crystal structure of R53 in complex with its epitope peptide using a novel
anti-C4 rabbit monoclonal antibody R53. Our data show that although the epitope
of R53 covers a highly conserved sequence (433)AMYAPPI(439), it is not available
in the gp120 trimer and in the CD4-bound conformation. Our results suggest a
masking mechanism to explain how HIV-1 protects this critical region from the
human immune system.
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