UniProt functional annotation for Q0P9D3

UniProt code: Q0P9D3.

Organism:		Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168).
Taxonomy:		Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Campylobacter.
Function:		Aminotransferase involved in the bacillosamine biosynthesis pathway by producing UDP-4-amino-4,6-dideoxy-alpha-D-GlcNAc (UDP-2- acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose), a precursor used in the production of the glycan component 2,4-diacetamido-2,4,6- trideoxy-alpha-D-glucopyranose. Required for host colonization and virulence. Involved in the N-linked protein glycosylation pathway. {ECO:0000269|PubMed:16286454, ECO:0000269|PubMed:16690622}.
Catalytic activity:		Reaction=2-oxoglutarate + UDP-N-acetylbacillosamine = L-glutamate + UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose; Xref=Rhea:RHEA:31663, ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:62375, ChEBI:CHEBI:63277; EC=2.6.1.34; Evidence={ECO:0000269|PubMed:16286454, ECO:0000269|PubMed:16690622};
Cofactor:		Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; Evidence={ECO:0000269|PubMed:16690622};
Biophysicochemical properties:		Kinetic parameters: KM=0.048 mM for UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D- glucosamine {ECO:0000269|PubMed:16286454, ECO:0000269|PubMed:16690622}; KM=0.61 mM for UDP-4-amino-4,6-dideoxy-alpha-D-N-acetyl-D-glucosamine {ECO:0000269|PubMed:16286454, ECO:0000269|PubMed:16690622}; Note=kcat is 144 min(-1) (PubMed:16286454). kcat is 14.9 min(-1) (PubMed:16690622). {ECO:0000269|PubMed:16286454, ECO:0000269|PubMed:16690622};
Pathway:		Protein modification; protein glycosylation.
Disruption phenotype:		Cells are impaired for flagella-mediated motility, for invasion of intestinal epithelial cells and for persistence in the chicken intestine. {ECO:0000269|PubMed:16690622}.
Similarity:		Belongs to the DegT/DnrJ/EryC1 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.