PDBsum entry 4ztc

Transferase

PDB id: 4ztc

Contents

Protein chain

385 a.a.

Ligands

4RA

Waters ×275

References listed in PDB file

Key reference

• Title: Structure of the external aldimine form of pgle, An aminotransferase required for n,N'-Diacetylbacillosamine biosynthesis.
• Authors: A.S.Riegert, N.M.Young, D.C.Watson, J.B.Thoden, H.M.Holden.
• Ref.: Protein Sci, 2015, 24, 1609-1616. [DOI no: 10.1002/pro.2745]
• PubMed id: 26178292

Abstract

N,N'-diacetylbacillosamine is a novel sugar that plays a key role in bacterial glycosylation. Three enzymes are required for its biosynthesis in Campylobacter jejuni starting from UDP-GlcNAc. The focus of this investigation, PglE, catalyzes the second step in the pathway. It is a PLP-dependent aminotransferase that converts UDP-2-acetamido-4-keto-2,4,6-trideoxy-d-glucose to UDP-2-acetamido-4-amino-2,4,6-trideoxy-d-glucose. For this investigation, the structure of PglE in complex with an external aldimine was determined to a nominal resolution of 2.0 Å. A comparison of its structure with those of other sugar aminotransferases reveals a remarkable difference in the manner by which PglE accommodates its nucleotide-linked sugar substrate.