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PDBsum entry 4zs3
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Fluorescent protein/inhibitor
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PDB id
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4zs3
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References listed in PDB file
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Key reference
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Title
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Fluorescein derivatives as bifunctional molecules for the simultaneous inhibiting and labeling of fto protein.
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Authors
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T.Wang,
T.Hong,
Y.Huang,
H.Su,
F.Wu,
Y.Chen,
L.Wei,
W.Huang,
X.Hua,
Y.Xia,
J.Xu,
J.Gan,
B.Yuan,
Y.Feng,
X.Zhang,
C.G.Yang,
X.Zhou.
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Ref.
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J Am Chem Soc, 2015,
137,
13736-13739.
[DOI no: ]
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PubMed id
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Abstract
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The FTO protein is unequivocally reported to play a critical role in human
obesity and in the regulation of cellular levels of m(6)A modification, which
makes FTO a significant and worthy subject of study. Here, we identified that
fluorescein derivatives can selectively inhibit FTO demethylation, and the
mechanisms behind these activities were elucidated after we determined the X-ray
crystal structures of FTO/fluorescein and FTO/5-aminofluorescein. Furthermore,
these inhibitors can also be applied to the direct labeling and enrichment of
FTO protein combined with photoaffinity labeling assay.
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