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UniProt functional annotation for P25910 | ||
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| UniProt code: P25910. |
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| Organism: | |
Bacteroides fragilis. |
| Taxonomy: | |
Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides. |
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| Function: | |
Confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring. {ECO:0000269|PubMed:2110145, ECO:0000269|PubMed:2121094, ECO:0000269|PubMed:9712862}. |
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| Catalytic activity: | |
Reaction=a beta-lactam + H2O = a substituted beta-amino acid; Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627, ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000269|PubMed:9712862}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:10210203, ECO:0000269|PubMed:12019104, ECO:0000269|PubMed:8805566, ECO:0000269|PubMed:9416622, ECO:0000269|PubMed:9545432, ECO:0000269|PubMed:9578564, ECO:0000269|PubMed:9712862, ECO:0000269|PubMed:9761816}; Note=Binds 2 Zn(2+) ions per subunit. It can also bind Cd(2+) and Co(2+) to a lesser extent (PubMed:9416622). {ECO:0000269|PubMed:12019104, ECO:0000269|PubMed:8805566, ECO:0000269|PubMed:9416622, ECO:0000269|PubMed:9545432, ECO:0000269|PubMed:9578564, ECO:0000269|PubMed:9712862, ECO:0000269|PubMed:9761816}; |
| Activity regulation: | |
Competitively inhibited by 4- morpholineethanesulfonic acid (MES), SB236050 and biphenyl tetrazoles (BPTs) (PubMed:9578564, PubMed:9545432, PubMed:12019104). Also inhibited by chelating agents such as EDTA and 1,10-phenanthroline (PubMed:9712862). CcrA is not susceptible to inactivation by the beta- lactamase-blocking agents clavulanic acid or tazobactam (PubMed:2121094, PubMed:2110145). {ECO:0000269|PubMed:12019104, ECO:0000269|PubMed:2110145, ECO:0000269|PubMed:2121094, ECO:0000269|PubMed:9545432, ECO:0000269|PubMed:9578564, ECO:0000269|PubMed:9712862}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=7.1 uM for nitrocefin (with zinc ions at pH 7 and 25 degrees Celsius) {ECO:0000269|PubMed:9712862}; KM=14 uM for nitrocefin (with cobalt ions at pH 7 and 25 degrees Celsius) {ECO:0000269|PubMed:9712862}; Note=Kcat is 225.7 sec(-1) for lactamase activity with nitrocefin as substrate (with zinc ions at pH 7 and 25 degrees Celsius). Kcat is 107.6 sec(-1) for lactamase activity with nitrocefin as substrate (with cobalt ions at pH 7 and 25 degrees Celsius). {ECO:0000269|PubMed:9712862}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:12019104, ECO:0000269|PubMed:9545432, ECO:0000269|PubMed:9578564, ECO:0000269|PubMed:9761816}. |
| Subcellular location: | |
Periplasm {ECO:0000269|PubMed:2110145}. |
| Similarity: | |
Belongs to the metallo-beta-lactamase superfamily. Class-B beta-lactamase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.