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UniProt functional annotation for Q06253 | ||
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| UniProt code: Q06253. |
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| Organism: | |
Escherichia phage P1 (Bacteriophage P1). |
| Taxonomy: | |
Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Punavirus. |
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| Function: | |
Antitoxin component of a type II toxin-antitoxin (TA) system (PubMed:18398006, PubMed:24141193, PubMed:18757857). A labile antitoxin that binds to cognate doc toxin and neutralizes its ability to phosphorylate host EF-Tu. Does not reverse phosphorylation. Bacteriophage P1 lysogenizes bacteria as a low-copy number plasmid; phd and doc proteins function in unison to stabilize plasmid number by inducing a lethal response to P1 plasmid prophage loss (PubMed:8411153). {ECO:0000269|PubMed:18398006, ECO:0000269|PubMed:18757857, ECO:0000269|PubMed:24141193, ECO:0000269|PubMed:8411153}. |
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| Function: | |
Binds to its own promoter repressing its expression; toxin doc acts as a corepressor or derepressor depending on the ratio, repressing or inducing expression. {ECO:0000269|PubMed:20603017, ECO:0000269|PubMed:9829946}. |
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| Subunit: | |
Homodimer. Interacts with cognate toxin doc, the exact ratio of doc:phd varies from 1:1 to 1:3. Interaction with doc prevents both kinase activity and dephosphorylation of EF-Tu. {ECO:0000269|PubMed:18398006, ECO:0000269|PubMed:18757857, ECO:0000269|PubMed:20696400, ECO:0000269|PubMed:9829946}. |
| Ptm: | |
Degraded by the ClpXP protease. {ECO:0000269|PubMed:7724551}. |
| Miscellaneous: | |
The concentration of phd in P1 lysogens is far greater than that of the toxin it antagonizes. Such an excess may assure the well-being of carriers of the addicting plasmid. {ECO:0000305|PubMed:8411153}. |
| Similarity: | |
Belongs to the phD/YefM antitoxin family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.