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PDBsum entry 4zkj
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Unknown function
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PDB id
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4zkj
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References listed in PDB file
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Key reference
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Title
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Crystal structure of streptococcus pyogenes cas1 and its interaction with csn2 in the type ii crispr-Cas system.
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Authors
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D.Ka,
H.Lee,
Y.D.Jung,
K.Kim,
C.Seok,
N.Suh,
E.Bae.
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Ref.
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Structure, 2016,
24,
70-79.
[DOI no: ]
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PubMed id
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Abstract
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CRISPRs and Cas proteins constitute an RNA-guided microbial immune system
against invading nucleic acids. Cas1 is a universal Cas protein found in all
three types of CRISPR-Cas systems, and its role is implicated in new spacer
acquisition during CRISPR-mediated adaptive immunity. Here, we report the
crystal structure of Streptococcus pyogenes Cas1 (SpCas1) in a type II
CRISPR-Cas system and characterize its interaction with S. pyogenes Csn2
(SpCsn2). The SpCas1 structure reveals a unique conformational state distinct
from type I Cas1 structures, resulting in a more extensive dimerization
interface, a more globular overall structure, and a disruption of potential
metal-binding sites for catalysis. We demonstrate that SpCas1 directly interacts
with SpCsn2, and identify the binding interface and key residues for Cas complex
formation. These results provide structural information for a type II Cas1
protein, and lay a foundation for studying multiprotein Cas complexes
functioning in type II CRISPR-Cas systems.
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