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PDBsum entry 4ziz
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Electron transport
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PDB id
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4ziz
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References listed in PDB file
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Key reference
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Title
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Serial femtosecond crystallography of soluble proteins in lipidic cubic phase.
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Authors
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R.Fromme,
A.Ishchenko,
M.Metz,
S.R.Chowdhury,
S.Basu,
S.Boutet,
P.Fromme,
T.A.White,
A.Barty,
J.C.Spence,
U.Weierstall,
W.Liu,
V.Cherezov.
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Ref.
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Iucrj, 2015,
2,
545-551.
[DOI no: ]
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PubMed id
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Abstract
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Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs)
enables high-resolution protein structure determination using micrometre-sized
crystals at room temperature with minimal effects from radiation damage. SFX
requires a steady supply of microcrystals intersecting the XFEL beam at random
orientations. An LCP-SFX method has recently been introduced in which
microcrystals of membrane proteins are grown and delivered for SFX data
collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic
phase (LCP), using a special LCP microextrusion injector. Here, it is
demonstrated that LCP can also be used as a suitable carrier medium for
microcrystals of soluble proteins, enabling a dramatic reduction in the amount
of crystallized protein required for data collection compared with crystals
delivered by liquid injectors. High-quality LCP-SFX data sets were collected for
two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each
protein.
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