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UniProt functional annotation for Q13822 | ||
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| UniProt code: Q13822. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids (PubMed:15769751, PubMed:26371182, PubMed:27754931, PubMed:14500380, PubMed:12354767,). Major substrate is lysophosphatidylcholine (PubMed:12176993, PubMed:27754931, PubMed:14500380). Also can act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility (PubMed:14500380). Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP (PubMed:15769751, PubMed:12176993). Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation (PubMed:11559573). Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein (PubMed:1733949). May have a role in induction of parturition (PubMed:12176993). Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor (PubMed:1733949, PubMed:11559573). Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids (PubMed:21393252). Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3- phosphocholine (lyso-PAF) (PubMed:21393252). {ECO:0000269|PubMed:11559573, ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:12354767, ECO:0000269|PubMed:14500380, ECO:0000269|PubMed:15769751, ECO:0000269|PubMed:1733949, ECO:0000269|PubMed:21240271, ECO:0000269|PubMed:21393252, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931, ECO:0000305|PubMed:15700135}. |
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| Catalytic activity: | |
Reaction=1-O-alkyl-sn-glycero-3-phosphoethanolamine + H2O = 1-O-alkyl- sn-glycero-3-phosphate + ethanolamine + H(+); Xref=Rhea:RHEA:15965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57603, ChEBI:CHEBI:58014, ChEBI:CHEBI:76168; EC=3.1.4.39; Evidence={ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:15769751, ECO:0000269|PubMed:18175805, ECO:0000269|PubMed:21240271, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931}; |
| Catalytic activity: | |
Reaction=1-O-(9Z-octadecenyl)-sn-glycero-3-phosphocholine + H2O = 1-O- (9Z-octadecenyl)-sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:41684, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:64396, ChEBI:CHEBI:78402; Evidence={ECO:0000269|PubMed:21393252}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41685; Evidence={ECO:0000305|PubMed:21393252}; |
| Catalytic activity: | |
Reaction=a 2-acyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn- glycerol 3-phosphate + choline + H(+); Xref=Rhea:RHEA:41712, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57875, ChEBI:CHEBI:64982; Evidence={ECO:0000269|PubMed:21393252}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41713; Evidence={ECO:0000305|PubMed:21393252}; |
| Catalytic activity: | |
Reaction=a 2-acyl-sn-glycero-3-phospho-L-serine + H2O = a 2-acyl-sn- glycerol 3-phosphate + H(+) + L-serine; Xref=Rhea:RHEA:41716, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33384, ChEBI:CHEBI:64982, ChEBI:CHEBI:65214; Evidence={ECO:0000269|PubMed:21393252}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41717; Evidence={ECO:0000305|PubMed:21393252}; |
| Catalytic activity: | |
Reaction=1-dodecanoyl-sn-glycero-3-phosphocholine + H2O = 1-dodecanoyl- sn-glycerol 3-phosphate + choline + H(+); Xref=Rhea:RHEA:38991, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:72682, ChEBI:CHEBI:74966; Evidence={ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:21240271}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38992; Evidence={ECO:0000305|PubMed:12176993, ECO:0000305|PubMed:21240271}; |
| Catalytic activity: | |
Reaction=H2O + sphing-4-enine-phosphocholine = choline + H(+) + sphing- 4-enine 1-phosphate; Xref=Rhea:RHEA:38919, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:58906, ChEBI:CHEBI:60119; Evidence={ECO:0000269|PubMed:14500380}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38920; Evidence={ECO:0000305|PubMed:14500380}; |
| Catalytic activity: | |
Reaction=a 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1- (9Z-octadecenoyl)-sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:38915, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28610, ChEBI:CHEBI:74544; Evidence={ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:14500380}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38916; Evidence={ECO:0000305|PubMed:12176993, ECO:0000305|PubMed:14500380}; |
| Catalytic activity: | |
Reaction=1-tetradecanoyl-sn-glycero-3-phosphocholine + H2O = 1- tetradecanoyl-sn-glycerol 3-phosphate + choline + H(+); Xref=Rhea:RHEA:38983, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:64489, ChEBI:CHEBI:72683; Evidence={ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:21240271}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38984; Evidence={ECO:0000305|PubMed:12176993, ECO:0000305|PubMed:21240271}; |
| Catalytic activity: | |
Reaction=1-decanoyl-sn-glycero-3-phosphocholine + H2O = 1-decanoyl-sn- glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:41131, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:77724, ChEBI:CHEBI:77726; Evidence={ECO:0000269|PubMed:12176993}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41132; Evidence={ECO:0000305|PubMed:12176993}; |
| Catalytic activity: | |
Reaction=1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1- hexadecanoyl-sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:38975, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57518, ChEBI:CHEBI:72998; Evidence={ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:21240271}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38976; Evidence={ECO:0000305|PubMed:12176993, ECO:0000305|PubMed:21240271}; |
| Catalytic activity: | |
Reaction=1-octadecanoyl-sn-glycero-3-phosphocholine + H2O = 1- octadecanoyl-sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:38979, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:73858, ChEBI:CHEBI:74565; Evidence={ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:21240271}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38980; Evidence={ECO:0000305|PubMed:12176993, ECO:0000305|PubMed:21240271}; |
| Catalytic activity: | |
Reaction=1-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphocholine + H2O = 1-(9Z,12Z)-octadecadienoyl-sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:41135, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28733, ChEBI:CHEBI:74547; Evidence={ECO:0000269|PubMed:12176993}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41136; Evidence={ECO:0000305|PubMed:12176993}; |
| Catalytic activity: | |
Reaction=1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:41139, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:74344, ChEBI:CHEBI:74938; Evidence={ECO:0000269|PubMed:12176993}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41140; Evidence={ECO:0000305|PubMed:12176993}; |
| Catalytic activity: | |
Reaction=1-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 1-O- hexadecyl-sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:41143, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:64496, ChEBI:CHEBI:77580; Evidence={ECO:0000269|PubMed:12176993}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41144; Evidence={ECO:0000305|PubMed:12176993}; |
| Catalytic activity: | |
Reaction=1-hexanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexanoyl-sn- glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:41400, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:78215, ChEBI:CHEBI:78223; Evidence={ECO:0000269|PubMed:12176993}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41401; Evidence={ECO:0000305|PubMed:12176993}; |
| Catalytic activity: | |
Reaction=1,2-dioctanoyl-sn-glycero-3-phosphocholine + H2O = 1,2- dioctanoyl-sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:41416, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:78228, ChEBI:CHEBI:78229; Evidence={ECO:0000269|PubMed:12176993}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41417; Evidence={ECO:0000305|PubMed:12176993}; |
| Catalytic activity: | |
Reaction=1,2-didecanoyl-sn-glycero-3-phosphocholine + H2O = 1,2- didecanoyl-sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:41412, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:78226, ChEBI:CHEBI:78227; Evidence={ECO:0000269|PubMed:12176993}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41413; Evidence={ECO:0000305|PubMed:12176993}; |
| Catalytic activity: | |
Reaction=1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine + H2O = 1-O-(1Z- alkenyl)-sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:41588, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:77283, ChEBI:CHEBI:77287; Evidence={ECO:0000269|PubMed:12176993}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:41589; Evidence={ECO:0000305|PubMed:12176993}; |
| Catalytic activity: | |
Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ethanolamine + H(+); Xref=Rhea:RHEA:38927, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57603, ChEBI:CHEBI:74544, ChEBI:CHEBI:74971; Evidence={ECO:0000250|UniProtKB:Q64610}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38928; Evidence={ECO:0000250|UniProtKB:Q64610}; |
| Catalytic activity: | |
Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phospho-L-serine + H2O = 1- (9Z-octadecenoyl)-sn-glycero-3-phosphate + H(+) + L-serine; Xref=Rhea:RHEA:38931, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33384, ChEBI:CHEBI:74544, ChEBI:CHEBI:74617; Evidence={ECO:0000250|UniProtKB:Q64610}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38932; Evidence={ECO:0000250|UniProtKB:Q64610}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931, ECO:0000269|PubMed:28414242}; Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931, ECO:0000269|PubMed:28414242}; |
| Cofactor: | |
Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931, ECO:0000269|PubMed:28414242}; Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931, ECO:0000269|PubMed:28414242}; |
| Activity regulation: | |
Inhibited by lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P). Inhibited by EDTA and EGTA (Probable). {ECO:0000305|PubMed:15769751, ECO:0000305|PubMed:18175805, ECO:0000305|PubMed:21240271}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.5 mM for 16:0-LPC (at pH 8.5) {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805}; KM=5.5 mM for pNP-TMP (at pH 8.5) {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805}; KM=11.3 mM for pNppp (isoform 1) {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805}; KM=5.7 mM for pNppp (isoform 2) {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805}; KM=19.8 mM for pNppp (isoform 3) {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805}; KM=96 uM for sn-1 lyso-PAF {ECO:0000269|PubMed:21393252}; KM=51 uM for sn-2 lyso-PAF {ECO:0000269|PubMed:21393252}; KM=0.10 mM for lysophosphatidylcholine {ECO:0000269|PubMed:14500380}; KM=0.23 mM for sphingosylphosphorylcholine {ECO:0000269|PubMed:14500380}; Vmax=1.9 nmol/min/ug enzyme with pNppp as substrate (isoform 1) {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805}; Vmax=0.67 nmol/min/ug enzyme with pNppp as substrate (isoform 2) {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805}; Vmax=1.6 nmol/min/ug enzyme with pNppp as substrate (isoform 3) {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805}; Vmax=0.11 umol/min/mg enzyme with sn-1 lyso-PAF as substrate {ECO:0000269|PubMed:21393252}; Vmax=0.025 umol/min/mg enzyme with sn-2 lyso-PAF as substrate {ECO:0000269|PubMed:21393252}; Vmax=11.8 nmol/min/ug enzyme with lysophosphatidylcholine as substrate {ECO:0000269|PubMed:14500380}; Vmax=6.1 nmol/min/ug enzyme with sphingosylphosphorylcholine as substrate {ECO:0000269|PubMed:14500380}; pH dependence: Optimum pH is 9.0 (isoform 1), 8.0 (isoform 3). Isoform 1 is less sensitive to pH. Isoform 1, isoform 2 and isoform 3 all retain some activity at pH 9.5. {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805}; Temperature dependence: Isoform 1 and isoform 3 are active from 45 to 60 degrees Celsius. {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805}; |
| Subcellular location: | |
Secreted {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:15769751, ECO:0000269|PubMed:1733949, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:27754931}. |
| Tissue specificity: | |
Detected in blood plasma (at protein level) (PubMed:12176993, PubMed:26371182). Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues. {ECO:0000269|PubMed:12176993, ECO:0000269|PubMed:18175805, ECO:0000269|PubMed:26371182, ECO:0000269|PubMed:8579579, ECO:0000269|PubMed:8586446}. |
| Induction: | |
Up-regulated in massively obese subjects with glucose intolerance, and during adipogenesis. {ECO:0000269|PubMed:15700135}. |
| Ptm: | |
N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. {ECO:0000250|UniProtKB:Q9R1E6}. |
| Ptm: | |
The interdomain disulfide bond between Cys-414 and Cys-806 is essential for catalytic activity. {ECO:0000250|UniProtKB:Q9R1E6}. |
| Similarity: | |
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.