 |
PDBsum entry 4zfs
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Fluorescent protein
|
PDB id
|
|
|
|
4zfs
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Crystal structure of phototoxic orange fluorescent proteins with a tryptophan-Based chromophore.
|
|
|
Authors
|
|
N.V.Pletneva,
V.Z.Pletnev,
K.S.Sarkisyan,
D.A.Gorbachev,
E.S.Egorov,
A.S.Mishin,
K.A.Lukyanov,
Z.Dauter,
S.Pletnev.
|
|
|
Ref.
|
|
Plos One, 2015,
10,
e0145740.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Abstract
|
|
|
Phototoxic fluorescent proteins represent a sparse group of genetically encoded
photosensitizers that could be used for precise light-induced inactivation of
target proteins, DNA damage, and cell killing. Only two such GFP-based
fluorescent proteins (FPs), KillerRed and its monomeric variant SuperNova, were
described up to date. Here, we present a crystallographic study of their two
orange successors, dimeric KillerOrange and monomeric mKillerOrange, at 1.81 and
1.57 Å resolution, respectively. They are the first orange-emitting protein
photosensitizers with a tryptophan-based chromophore (Gln65-Trp66-Gly67). Same
as their red progenitors, both orange photosensitizers have a water-filled
channel connecting the chromophore to the β-barrel exterior and enabling
transport of ROS. In both proteins, Trp66 of the chromophore adopts an unusual
trans-cis conformation stabilized by H-bond with the nearby Gln159. This
trans-cis conformation along with the water channel was shown to be a key
structural feature providing bright orange emission and phototoxicity of both
examined orange photosensitizers.
|
|
|
|
|
|
|
