PDBsum entry 4zbc

Isomerase

PDB id: 4zbc

Contents

Protein chains

387 a.a.

Ligands

GLC ×2

FRU ×4

GLC-GLC

Metals

_MN ×11

Waters ×542

PDB id:

4zbc

Name:

Isomerase

Title:

A dehydrated form of glucose isomerase collected at 100k.

Structure:

Xylose isomerase. Chain: a, b. Synonym: glucose isomerase. Engineered: yes

Source:

Streptomyces rubiginosus. Organism_taxid: 1929. Gene: xyla. Expressed in: streptomyces rubiginosus. Expression_system_taxid: 1929

Resolution:

2.00Å R-factor: 0.138 R-free: 0.182

Authors:

J.Sandy

Key ref:

C.M.Lobley et al. (2016). A generic protocol for protein crystal dehydration using the HC1b humidity controller. Acta Crystallogr D Struct Biol, 72, 629-640. PubMed id: 27139626 DOI: 10.1107/S2059798316003065

Date:

14-Apr-15 Release date: 23-Mar-16

Protein chains

P24300  (XYLA_STRRU) -  Xylose isomerase from Streptomyces rubiginosus

Seq: 388 a.a.

Struc: 387 a.a.

Enzyme reactions

• Enzyme class: E.C.5.3.1.5 - xylose isomerase.
• Reaction: alpha-D-xylose = alpha-D-xylulofuranose

alpha-D-xylose (Bound ligand (Het Group name = GLC) matches with 83.33% similarity) = alpha-D-xylulofuranose

• Cofactor: Mg(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Key reference

DOI no: 10.1107/S2059798316003065

Acta Crystallogr D Struct Biol 72:629-640 (2016)

PubMed id: 27139626

A generic protocol for protein crystal dehydration using the HC1b humidity controller.

C.M.Lobley, J.Sandy, J.Sanchez-Weatherby, M.Mazzorana, T.Krojer, R.P.Nowak, T.L.Sorensen.

ABSTRACT

Dehydration may change the crystal lattice and affect the mosaicity, resolution and quality of X-ray diffraction data. A dehydrating environment can be generated around a crystal in several ways with various degrees of precision and complexity. This study uses a high-precision crystal humidifier/dehumidifier to provide an airstream of known relative humidity in which the crystals are mounted: a precise yet hassle-free approach to altering crystal hydration. A protocol is introduced to assess the impact of crystal dehydration systematically applied to nine experimental crystal systems. In one case, that of glucose isomerase, dehydration triggering a change of space group from I222 to P21212 was observed. This observation is supported by an extended study of the behaviour of the glucose isomerase crystal structure during crystal dehydration.