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PDBsum entry 4z9e
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DNA binding protein
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PDB id
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4z9e
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References listed in PDB file
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Key reference
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Title
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Alba from thermoplasma volcanium belongs to α-Nat'S: an insight into the structural aspects of tv alba and its acetylation by tv ard1.
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Authors
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C.Ma,
C.Pathak,
S.J.Lee,
K.Y.Lee,
S.B.Jang,
M.Nam,
H.Im,
H.J.Yoon,
B.J.Lee.
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Ref.
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Arch Biochem Biophys, 2016,
590,
90.
[DOI no: ]
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PubMed id
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Abstract
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The Alba superfamily proteins have been regarded as a conserved group of
proteins in archaea and eukarya, which have shown to be important in nucleic
acid binding, chromatic organization and gene regulation. These proteins often
belong to the N-acetyltransferase (NAT) category (N(α)-acetyltransferases or
N(ε)-acetyltransferases) and undergo post-translational modifications. Here, we
report the crystal structure of Alba from Thermoplasma volcanium (Tv Alba) at
2.4 Å resolution. The acetylation of Tv Alba was monitored and the N-terminal
of Tv Alba has been shown to interact with acetyl coenzyme A (Ac-CoA). The
chemical shift perturbation experiments of Tv Alba were performed in the
presence of Ac-CoA and/or Tv Ard1, another T. volcanium protein that treats Tv
Alba as a substrate. To examine the DNA binding capabilities of Tv Alba alone
and in the presence of Ac-CoA and/or Tv Ard1, EMSA experiments were carried out.
It is shown that although Tv Alba binds to Ac-CoA, the acetylation of Tv Alba is
not related with its binding to dsDNA, and the involvement of the N-terminus in
Ac-CoA binding demonstrates that Tv Alba belongs to the N(α)-acetyltransferase
family.
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