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PDBsum entry 4z8u
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Protein binding
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PDB id
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4z8u
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References listed in PDB file
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Key reference
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Title
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Crystal structure of xanthomonas avrrxo1-Orf1, A type III effector with a polynucleotide kinase domain, And its interactor avrrxo1-Orf2.
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Authors
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Q.Han,
C.Zhou,
S.Wu,
Y.Liu,
L.Triplett,
J.Miao,
J.Tokuhisa,
L.Deblais,
H.Robinson,
J.E.Leach,
J.Li,
B.Zhao.
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Ref.
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Structure, 2015,
23,
1900-1909.
[DOI no: ]
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PubMed id
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Abstract
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Xanthomonas oryzae pv. oryzicola (Xoc) causes bacterial leaf streak (BLS)
disease on rice plants. Xoc delivers a type III effector AvrRxo1-ORF1 into rice
plant cells that can be recognized by disease resistance (R) protein Rxo1, and
triggers resistance to BLS disease. However, the mechanism and virulence role of
AvrRxo1 is not known. In the genome of Xoc, AvrRxo1-ORF1 is adjacent to another
gene AvrRxo1-ORF2, which was predicted to encode a molecular chaperone of
AvrRxo1-ORF1. We report the co-purification and crystallization of the
AvrRxo1-ORF1:AvrRxo1-ORF2 tetramer complex at 1.64 Å resolution. AvrRxo1-ORF1
has a T4 polynucleotide kinase domain, and expression of AvrRxo1-ORF1 suppresses
bacterial growth in a manner dependent on the kinase motif. Although
AvrRxo1-ORF2 binds AvrRxo1-ORF1, it is structurally different from typical
effector-binding chaperones, in that it has a distinct fold containing a novel
kinase-binding domain. AvrRxo1-ORF2 functions to suppress the bacteriostatic
activity of AvrRxo1-ORF1 in bacterial cells.
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