UniProt functional annotation for P08902



	UniProt functional annotation for P08902

UniProt code: P08902.

Organism: Dioclea grandiflora (Mucana).

Taxonomy: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Macropsychanthus.

Function: D-mannose/D-glucose-binding lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 50 ug/ml. {ECO:0000269|PubMed:10747944, ECO:0000269|PubMed:1398779, ECO:0000269|PubMed:18472821, ECO:0000269|PubMed:19765980, ECO:0000269|PubMed:7524287, ECO:0000269|PubMed:9575151}.

Subunit: Homotetramer. {ECO:0000269|PubMed:8812975, ECO:0000269|PubMed:9830028}.

Ptm: The beta and gamma chains are produced by partial proteolytic processing of the lectin alpha chain by an asparaginyl endopeptidase. Mixture of 60% alpha lectin and 40% of its beta and gamma proteolytic fragments.

Mass spectrometry: [Lectin alpha chain]: Mass=26602; Mass_error=4; Method=Electrospray; Evidence={ECO:0000269|PubMed:10082964};

Mass spectrometry: [Lectin beta chain]: Mass=12872; Mass_error=2; Method=Electrospray; Evidence={ECO:0000269|PubMed:10082964};

Mass spectrometry: [Lectin gamma chain]: Mass=12752; Mass_error=2; Method=Electrospray; Evidence={ECO:0000269|PubMed:10082964};

Toxic dose: LC(50) is 2.52 ug/ml against the brine shrimp A.salina. {ECO:0000269|PubMed:19765980}.

Miscellaneous: Binds one manganese (or another transition metal) ion and one calcium ion. The metal ions are essential for the saccharide- binding and cell-agglutinating activities.

Miscellaneous: Is being tested as a molluscicide with potential application in controlling schistosomiasis. The causative agent of schistosomiasis depends on freshwater snails of the genus Biomphalaria as hosts during its larval stages.

Similarity: Belongs to the leguminous lectin family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Dioclea grandiflora (Mucana).
Taxonomy:		Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Macropsychanthus.



Function:		D-mannose/D-glucose-binding lectin. Has anti-inflammatory activity in rats. Induces histamine release in mast cells from rat. Induces lymphocyte proliferation and IFNG production. Shows toxicity against the aquatic snail B.glabrata at concentrations higher than 50 ug/ml. {ECO:0000269\|PubMed:10747944, ECO:0000269\|PubMed:1398779, ECO:0000269\|PubMed:18472821, ECO:0000269\|PubMed:19765980, ECO:0000269\|PubMed:7524287, ECO:0000269\|PubMed:9575151}.


Subunit:		Homotetramer. {ECO:0000269\|PubMed:8812975, ECO:0000269\|PubMed:9830028}.
Ptm:		The beta and gamma chains are produced by partial proteolytic processing of the lectin alpha chain by an asparaginyl endopeptidase. Mixture of 60% alpha lectin and 40% of its beta and gamma proteolytic fragments.
Mass spectrometry:		[Lectin alpha chain]: Mass=26602; Mass_error=4; Method=Electrospray; Evidence={ECO:0000269\|PubMed:10082964};
Mass spectrometry:		[Lectin beta chain]: Mass=12872; Mass_error=2; Method=Electrospray; Evidence={ECO:0000269\|PubMed:10082964};
Mass spectrometry:		[Lectin gamma chain]: Mass=12752; Mass_error=2; Method=Electrospray; Evidence={ECO:0000269\|PubMed:10082964};
Toxic dose:		LC(50) is 2.52 ug/ml against the brine shrimp A.salina. {ECO:0000269\|PubMed:19765980}.
Miscellaneous:		Binds one manganese (or another transition metal) ion and one calcium ion. The metal ions are essential for the saccharide- binding and cell-agglutinating activities.
Miscellaneous:		Is being tested as a molluscicide with potential application in controlling schistosomiasis. The causative agent of schistosomiasis depends on freshwater snails of the genus Biomphalaria as hosts during its larval stages.
Similarity:		Belongs to the leguminous lectin family. {ECO:0000305}.