PDBsum entry 4z46

Hydrolase

PDB id: 4z46

Contents

Protein chain

129 a.a.

Ligands

EDO ×2

NO3 ×9

1PT

CPT

Metals

_NA ×2

Waters ×134

References listed in PDB file

Key reference

• Title: Oxaliplatin vs. Cisplatin: competition experiments on their binding to lysozyme.
• Authors: D.Marasco, L.Messori, T.Marzo, A.Merlino.
• Ref.: Dalton Trans, 2015, 44, 10392-10398. [DOI no: 10.1039/c5dt01279a]
• PubMed id: 25974859

Abstract

The model protein hen egg white lysozyme was challenged with oxaliplatin and cisplatin. ESI mass spectrometry, surface plasmon resonance and thermal shift analyses demonstrate the formation of a bis-platinum adduct, though in very small amounts. Crystals of the bis-platinum adduct were obtained using two different preparations and the X-ray structures were solved at 1.85 Å and 1.95 Å resolution. Overall, the obtained data point out that, under the analyzed conditions, the two Pt drugs have similar affinities for the protein, but bind on its surface at two non-overlapping sites. In other words, these two drugs manifest a significantly different reactivity with this model protein and do not compete for the same protein binding sites.