|
|
||
|
|
|
|
|
UniProt functional annotation for P95480 | ||
|
|
|
|
|
|
||
| UniProt code: P95480. |
|
||
| Organism: | |
Pseudomonas fluorescens. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. |
|
||
|
||
| Function: | |
Involved in the biosynthesis of the antifungal antibiotic pyrrolnitrin. Catalyzes the chlorination of tryptophan (Trp) at C7 position to yield 7-chloro-L-tryptophan (7-CLT). The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp. {ECO:0000269|PubMed:10941070, ECO:0000269|PubMed:9172332, ECO:0000269|PubMed:9537395}. |
|
||
| Catalytic activity: | |
Reaction=chloride + FADH2 + L-tryptophan + O2 = 7-chloro-L-tryptophan + FAD + 2 H2O; Xref=Rhea:RHEA:26494, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:17996, ChEBI:CHEBI:57692, ChEBI:CHEBI:57912, ChEBI:CHEBI:58307, ChEBI:CHEBI:58713; EC=1.14.19.9; Evidence={ECO:0000269|PubMed:9537395}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=17.1 uM for tryptophan (with FAD at pH 7.2 and 30 degrees Celsius) {ECO:0000269|PubMed:18979475}; Vmax=43 pmol/min/mg enzyme {ECO:0000269|PubMed:18979475}; |
| Pathway: | |
Antibiotic biosynthesis. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:16195462, ECO:0000269|PubMed:18979475}. |
| Disruption phenotype: | |
Cells lacking this gene lose the ability to produce pyrrolnitrin. {ECO:0000269|PubMed:9172332, ECO:0000269|PubMed:9537395}. |
| Similarity: | |
Belongs to the flavin-dependent halogenase family. Bacterial tryptophan halogenase subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.