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PDBsum entry 4z44
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Oxidoreductase
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PDB id
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4z44
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References listed in PDB file
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Key reference
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Title
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Extending the biocatalytic scope of regiocomplementary flavin-Dependent halogenase enzymes.
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Authors
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S.A.Shepherd,
C.Karthikeyan,
J.Latham,
A.W.Struck,
M.L.Thompson,
B.R.K.Menon,
M.Q.Styles,
C.Levy,
D.Leys,
J.Micklefield.
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Ref.
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Chem Sci, 2015,
6,
3454-3460.
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PubMed id
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Abstract
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Flavin-dependent halogenases are potentially valuable biocatalysts for the
regioselective halogenation of aromatic compounds. These enzymes, utilising
benign inorganic halides, offer potential advantages over traditional
non-enzymatic halogenation chemistry that often lacks regiocontrol and requires
deleterious reagents. Here we extend the biocatalytic repertoire of the
tryptophan halogenases, demonstrating how these enzymes can halogenate a range
of alternative aryl substrates. Using structure guided mutagenesis we also show
that it is possible to alter the regioselectivity as well as increase the
activity of the halogenases with non-native substrates including anthranilic
acid; an important intermediate in the synthesis and biosynthesis of
pharmaceuticals and other valuable products.
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