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PDBsum entry 4z3u
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Viral protein
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PDB id
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4z3u
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References listed in PDB file
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Key reference
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Title
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Structural basis of membrane budding by the nuclear egress complex of herpesviruses.
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Authors
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J.M.Bigalke,
E.E.Heldwein.
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Ref.
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Embo J, 2015,
34,
2921-2936.
[DOI no: ]
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PubMed id
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Abstract
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During nuclear egress, herpesvirus capsids bud at the inner nuclear membrane
forming perinuclear viral particles that subsequently fuse with the outer
nuclear membrane, releasing capsids into the cytoplasm. This unusual budding
process is mediated by the nuclear egress complex (NEC) composed of two
conserved viral proteins, UL31 and UL34. Earlier, we discovered that the
herpesvirus nuclear egress complex (NEC) could bud synthetic membranes in vitro
without the help of other proteins by forming a coat-like hexagonal scaffold
inside the budding membrane. To understand the structural basis of NEC-mediated
membrane budding, we determined the crystal structures of the NEC from two
herpesviruses. The hexagonal lattice observed in the NEC crystals recapitulates
the honeycomb coats within the budded vesicles. Perturbation of the oligomeric
interfaces through mutagenesis blocks budding in vitro confirming that NEC
oligomerization into a honeycomb lattice drives budding. The structure
represents the first atomic-level view of an oligomeric array formed by a
membrane-deforming protein, making possible the dissection of its unique
budding mechanism and the design of inhibitors to block it.
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