UniProt functional annotation for O69230



	UniProt functional annotation for O69230

UniProt code: O69230.

Organism: Paenibacillus barcinonensis.

Taxonomy: Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.

Function: Endoxylanase with high hydrolytic activity on birchwood and oat spelt xylan. Xylotetraose, xylotriose, xylobiose and xylose are the main products from birchwood xylan hydrolysis. Shows increasing activity on xylo-oligosaccharides of increasing length. Displays very low hydrolytic activity on Avicel, carboxymethylcellulose (CMC) and p- nitrophenyl-beta-xylopyranoside. Also shows transxylosidase activity, allowing the formation of xylo-oligosaccharides of higher degree of polymerization than the starting substrate. {ECO:0000269|PubMed:10463183}.

Catalytic activity: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|PubMed:10463183};

Biophysicochemical properties: pH dependence: Optimum pH is 5. Retains at least 50% of its maximum activity between pH 4.5 and 8.0. Is not active at pH values below 4.5, while at least 35% of maximum activity is found in the pH range 8.5-11.0. {ECO:0000269|PubMed:10463183}; Temperature dependence: Optimum temperature is 45 degrees Celsius. Is only stable at 55 degrees Celsius or lower temperatures. Retains more than 77% activity after 2 hours incubation at 55 degrees Celsius and pH 7. {ECO:0000269|PubMed:10463183};

Pathway: Glycan degradation; xylan degradation.

Domain: Consists of three different domains. The central region of the enzyme is the catalytic domain. The N-terminal region contains cenC- type cellulose-binding domains and seems to act as a thermostabilizing domain: a derivative lacking this region shows a lower optimum temperature for activity (35 degrees Celsius) than the full-length enzyme, and a reduced thermal stability that results in a complete inactivation of the enzyme after 2 hours incubation at 55 degrees Celsius. The C-terminal region contains family 9 carbohydrate-binding modules.

Similarity: Belongs to the glycosyl hydrolase 10 (cellulase F) family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Paenibacillus barcinonensis.
Taxonomy:		Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.



Function:		Endoxylanase with high hydrolytic activity on birchwood and oat spelt xylan. Xylotetraose, xylotriose, xylobiose and xylose are the main products from birchwood xylan hydrolysis. Shows increasing activity on xylo-oligosaccharides of increasing length. Displays very low hydrolytic activity on Avicel, carboxymethylcellulose (CMC) and p- nitrophenyl-beta-xylopyranoside. Also shows transxylosidase activity, allowing the formation of xylo-oligosaccharides of higher degree of polymerization than the starting substrate. {ECO:0000269\|PubMed:10463183}.


Catalytic activity:		Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269\|PubMed:10463183};
Biophysicochemical properties:		pH dependence: Optimum pH is 5. Retains at least 50% of its maximum activity between pH 4.5 and 8.0. Is not active at pH values below 4.5, while at least 35% of maximum activity is found in the pH range 8.5-11.0. {ECO:0000269\|PubMed:10463183}; Temperature dependence: Optimum temperature is 45 degrees Celsius. Is only stable at 55 degrees Celsius or lower temperatures. Retains more than 77% activity after 2 hours incubation at 55 degrees Celsius and pH 7. {ECO:0000269\|PubMed:10463183};
Pathway:		Glycan degradation; xylan degradation.
Domain:		Consists of three different domains. The central region of the enzyme is the catalytic domain. The N-terminal region contains cenC- type cellulose-binding domains and seems to act as a thermostabilizing domain: a derivative lacking this region shows a lower optimum temperature for activity (35 degrees Celsius) than the full-length enzyme, and a reduced thermal stability that results in a complete inactivation of the enzyme after 2 hours incubation at 55 degrees Celsius. The C-terminal region contains family 9 carbohydrate-binding modules.
Similarity:		Belongs to the glycosyl hydrolase 10 (cellulase F) family. {ECO:0000305}.