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PDBsum entry 4xtd
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Oxidoreductase
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PDB id
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4xtd
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References listed in PDB file
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Key reference
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Title
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Increased riboflavin production by manipulation of inosine 5'-Monophosphate dehydrogenase in ashbya gossypii.
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Authors
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R.M.Buey,
R.Ledesma-Amaro,
M.Balsera,
J.M.De pereda,
J.L.Revuelta.
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Ref.
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Appl Microbiol Biotechnol, 2015,
99,
9577-9589.
[DOI no: ]
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PubMed id
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Abstract
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Guanine nucleotides are the precursors of essential biomolecules including
nucleic acids and vitamins such as riboflavin. The enzyme
inosine-5'-monophosphate dehydrogenase (IMPDH) catalyzes the ratelimiting step
in the guanine nucleotide de novo biosynthetic pathway and plays a key role in
controlling the cellular nucleotide pools. Thus, IMPDH is an important metabolic
bottleneck in the guanine nucleotide synthesis, susceptible of manipulation by
means of metabolic engineering approaches. Herein, we report the functional and
structural characterization of the IMPDH enzyme from the industrial fungus
Ashbya gossypii. Our data show that the overexpression of the IMPDH gene
increases the metabolic flux through the guanine pathway and ultimately enhances
40 % riboflavin production with respect to the wild type. Also, IMPDH disruption
results in a 100-fold increase of inosine excretion to the culture media. Our
results contribute to the developing metabolic engineering toolbox aiming at
improving the production of metabolites with biotechnological interest in A.
gossypii.
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