UniProt functional annotation for Q7K4Y6



	UniProt functional annotation for Q7K4Y6

UniProt code: Q7K4Y6.

Organism: Drosophila melanogaster (Fruit fly).

Taxonomy: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.

Function: Sodium-dependent dopamine transporter which terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (PubMed:11125028, PubMed:12606774, PubMed:24037379, PubMed:25970245). Also transports tyramine and norepinephrine, shows less efficient transport of octopamine and does not transport serotonin (PubMed:11125028, PubMed:12606774). Plays a role in the regulation of the rest/activity cycle (PubMed:16093388, PubMed:25232310). {ECO:0000269|PubMed:11125028, ECO:0000269|PubMed:12606774, ECO:0000269|PubMed:16093388, ECO:0000269|PubMed:24037379, ECO:0000269|PubMed:25232310, ECO:0000269|PubMed:25970245}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:24037379, ECO:0000269|PubMed:25970245}; Multi-pass membrane protein {ECO:0000269|PubMed:24037379, ECO:0000269|PubMed:25970245}.

Tissue specificity: Expression is restricted to dopaminergic neurons in the central nervous system. {ECO:0000269|PubMed:11125028}.

Developmental stage: Low levels in 0-4 hour embryos with strong expression in late embryos at 12-24 hours and during larval development. Expression decreases during pupal development and increases again in adult flies with heads showing higher levels than bodies. {ECO:0000269|PubMed:11125028}.

Disruption phenotype: No effect on fertility or longevity but mutants display longer periods of daily activity than controls, reduced rest, enhanced sensitivity to mechanical stimuli when inactive and decreased rest rebound in response to rest deprivation (PubMed:16093388). Impaired aversive olfactory memory due to excessive dopaminergic signaling (PubMed:25232310). RNAi-mediated knockdown in neurons causes significant reduction in the sleep-like rest state with total daily resting periods decreased to about half of that of control flies (PubMed:25232310). {ECO:0000269|PubMed:16093388, ECO:0000269|PubMed:25232310}.

Miscellaneous: 10-fold less sensitive to cocaine than mammalian dopamine transporter SLC6A3. {ECO:0000269|PubMed:12606774}.

Similarity: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. {ECO:0000255|RuleBase:RU003732}.

Annotations taken from UniProtKB at the EBI.


Organism:		Drosophila melanogaster (Fruit fly).
Taxonomy:		Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.



Function:		Sodium-dependent dopamine transporter which terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (PubMed:11125028, PubMed:12606774, PubMed:24037379, PubMed:25970245). Also transports tyramine and norepinephrine, shows less efficient transport of octopamine and does not transport serotonin (PubMed:11125028, PubMed:12606774). Plays a role in the regulation of the rest/activity cycle (PubMed:16093388, PubMed:25232310). {ECO:0000269\|PubMed:11125028, ECO:0000269\|PubMed:12606774, ECO:0000269\|PubMed:16093388, ECO:0000269\|PubMed:24037379, ECO:0000269\|PubMed:25232310, ECO:0000269\|PubMed:25970245}.


Subcellular location:		Cell membrane {ECO:0000269\|PubMed:24037379, ECO:0000269\|PubMed:25970245}; Multi-pass membrane protein {ECO:0000269\|PubMed:24037379, ECO:0000269\|PubMed:25970245}.
Tissue specificity:		Expression is restricted to dopaminergic neurons in the central nervous system. {ECO:0000269\|PubMed:11125028}.
Developmental stage:		Low levels in 0-4 hour embryos with strong expression in late embryos at 12-24 hours and during larval development. Expression decreases during pupal development and increases again in adult flies with heads showing higher levels than bodies. {ECO:0000269\|PubMed:11125028}.
Disruption phenotype:		No effect on fertility or longevity but mutants display longer periods of daily activity than controls, reduced rest, enhanced sensitivity to mechanical stimuli when inactive and decreased rest rebound in response to rest deprivation (PubMed:16093388). Impaired aversive olfactory memory due to excessive dopaminergic signaling (PubMed:25232310). RNAi-mediated knockdown in neurons causes significant reduction in the sleep-like rest state with total daily resting periods decreased to about half of that of control flies (PubMed:25232310). {ECO:0000269\|PubMed:16093388, ECO:0000269\|PubMed:25232310}.
Miscellaneous:		10-fold less sensitive to cocaine than mammalian dopamine transporter SLC6A3. {ECO:0000269\|PubMed:12606774}.
Similarity:		Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. {ECO:0000255\|RuleBase:RU003732}.