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UniProt functional annotation for P9WP79 | ||
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| UniProt code: P9WP79. |
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| Organism: | |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. |
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| Function: | |
Bifunctional enzyme that catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L- lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives, and the FMNH2- dependent reduction of dehydro-F420-0 to form F420-0. {ECO:0000255|HAMAP-Rule:MF_01259, ECO:0000269|PubMed:26861878, ECO:0000269|PubMed:30952857}. |
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| Catalytic activity: | |
Reaction=GTP + L-glutamate + oxidized coenzyme F420-0 = GDP + H(+) + oxidized coenzyme F420-1 + phosphate; Xref=Rhea:RHEA:30555, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189, ChEBI:CHEBI:59907, ChEBI:CHEBI:59920; EC=6.3.2.31; Evidence={ECO:0000255|HAMAP- Rule:MF_01259, ECO:0000269|PubMed:26861878}; |
| Catalytic activity: | |
Reaction=GTP + L-glutamate + oxidized coenzyme F420-1 = GDP + H(+) + oxidized coenzyme F420-2 + phosphate; Xref=Rhea:RHEA:30523, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:57922, ChEBI:CHEBI:58189, ChEBI:CHEBI:59920; EC=6.3.2.34; Evidence={ECO:0000255|HAMAP- Rule:MF_01259, ECO:0000269|PubMed:26861878}; |
| Catalytic activity: | |
Reaction=GTP + L-glutamate + oxidized coenzyme F420-(gamma-Glu)(n) = GDP + H(+) + oxidized coenzyme F420-(gamma-Glu)(n+1) + phosphate; Xref=Rhea:RHEA:51236, Rhea:RHEA-COMP:12939, Rhea:RHEA-COMP:12940, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189, ChEBI:CHEBI:133980; Evidence={ECO:0000255|HAMAP-Rule:MF_01259, ECO:0000269|PubMed:26861878}; |
| Catalytic activity: | |
Reaction=FMN + H(+) + oxidized coenzyme F420-0 = dehydro coenzyme F420- 0 + FMNH2; Xref=Rhea:RHEA:60360, ChEBI:CHEBI:15378, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:59907, ChEBI:CHEBI:143705; Evidence={ECO:0000269|PubMed:30952857}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_01259}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP-Rule:MF_01259}; Note=Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese. {ECO:0000255|HAMAP-Rule:MF_01259}; |
| Cofactor: | |
Name=K(+); Xref=ChEBI:CHEBI:29103; Evidence={ECO:0000255|HAMAP-Rule:MF_01259}; Note=Monovalent cation. The ion could be potassium. {ECO:0000255|HAMAP- Rule:MF_01259}; |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:26861878}; |
| Pathway: | |
Cofactor biosynthesis; coenzyme F420 biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01259}. |
| Similarity: | |
In the N-terminal section; belongs to the CofE family. {ECO:0000255|HAMAP-Rule:MF_01259}. |
Annotations taken from UniProtKB at the EBI.