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PDBsum entry 4xkp
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Transport protein
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PDB id
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4xkp
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References listed in PDB file
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Key reference
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Title
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Novel insights into nickel import in staphylococcus aureus: the positive role of free histidine and structural characterization of a new thiazolidine-Type nickel chelator.
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Authors
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H.Lebrette,
E.Borezée-Durant,
L.Martin,
P.Richaud,
E.Boeri erba,
C.Cavazza.
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Ref.
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Metallomics, 2015,
7,
613-621.
[DOI no: ]
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PubMed id
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Abstract
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Staphylococcus aureus possesses two canonical ABC-importers dedicated to nickel
acquisition: the NikABCDE and the CntABCDF systems, active under different
growth conditions. This study reports on the extracytoplasmic nickel-binding
components SaNikA and SaCntA. We showed by protein crystallography that SaNikA
is able to bind either a Ni-(l-His)2 complex or a Ni-(l-His)
(2-methyl-thiazolidine dicarboxylate) complex, depending on their availability
in culture supernatants. Native mass spectrometry experiments on SaCntA revealed
that it binds the Ni(ii) ion via a different histidine-dependent chelator but it
cannot bind Ni-(l-His)2. In vitro experiments are consistent with in vivo nickel
content measurements that showed that l-histidine has a high positive impact on
nickel import via the Cnt system. These results suggest that although both
systems may require free histidine, they use different strategies to import
nickel.
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