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PDBsum entry 4xhc
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References listed in PDB file
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Key reference
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Title
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Crystal structure of a novel two domain gh78 family α-Rhamnosidase from klebsiella oxytoca with rhamnose bound.
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Authors
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E.C.O'Neill,
C.E.Stevenson,
M.J.Paterson,
M.Rejzek,
A.L.Chauvin,
D.M.Lawson,
R.A.Field.
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Ref.
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Proteins, 2015,
83,
1742-1749.
[DOI no: ]
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PubMed id
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Abstract
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The crystal structure of the GH78 family α-rhamnosidase from Klebsiella oxytoca
(KoRha) has been determined at 2.7 Å resolution with rhamnose bound in the
active site of the catalytic domain. Curiously, the putative catalytic acid, Asp
222, is preceded by an unusual non-proline cis-peptide bond which helps to
project the carboxyl group into the active centre. This KoRha homodimeric
structure is significantly smaller than those of the other previously determined
GH78 structures. Nevertheless, the enzyme displays α-rhamnosidase activity when
assayed in vitro, suggesting that the additional structural domains found in the
related enzymes are dispensible for function. Proteins 2015; 83:1742-1749. ©
2015 The Authors. Proteins: Structure, Function, and Bioinformatics Published by
Wiley Periodicals, Inc.
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