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PDBsum entry 4xfx
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Viral protein
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PDB id
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4xfx
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References listed in PDB file
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Key reference
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Title
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Structural virology. X-Ray crystal structures of native HIV-1 capsid protein reveal conformational variability.
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Authors
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A.T.Gres,
K.A.Kirby,
V.N.Kewalramani,
J.J.Tanner,
O.Pornillos,
S.G.Sarafianos.
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Ref.
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Science, 2015,
349,
99.
[DOI no: ]
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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The detailed molecular interactions between native HIV-1 capsid protein (CA)
hexamers that shield the viral genome and proteins have been elusive. We report
crystal structures describing interactions between CA monomers related by
sixfold symmetry within hexamers (intrahexamer) and threefold and twofold
symmetry between neighboring hexamers (interhexamer). The structures describe
how CA builds hexagonal lattices, the foundation of mature capsids. Lattice
structure depends on an adaptable hydration layer modulating interactions among
CA molecules. Disruption of this layer alters interhexamer interfaces,
highlighting an inherent structural variability. A CA-targeting antiviral
affects capsid stability by binding across CA molecules and subtly altering
interhexamer interfaces remote to the ligand-binding site. Inherent structural
plasticity, hydration layer rearrangement, and effector binding affect capsid
stability and have functional implications for the retroviral life cycle.
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