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PDBsum entry 4x9d
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RNA binding protein
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PDB id
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4x9d
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References listed in PDB file
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Key reference
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Title
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Characterization of RNA-Binding properties of the archaeal hfq-Like protein from methanococcus jannaschii.
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Authors
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A.Nikulin,
A.Mikhailina,
N.Lekontseva,
V.Balobanov,
E.Nikonova,
S.Tishchenko.
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Ref.
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J Biomol Struct Dyn, 2017,
35,
1615-1628.
[DOI no: ]
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PubMed id
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Abstract
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The Sm and Sm-like proteins are widely distributed among bacteria, archaea and
eukarya. They participate in many processes related to RNA-processing and
regulation of gene expression. While the function of the bacterial Lsm protein
Hfq and eukaryotic Sm/Lsm proteins is rather well studied, the role of Lsm
proteins in Archaea is investigated poorly. In this work, the RNA-binding
ability of an archaeal Hfq-like protein from Methanococcus jannaschii has been
studied by X-ray crystallography, anisotropy fluorescence and surface plasmon
resonance. It has been found that MjaHfq preserves the proximal RNA-binding site
that usually recognizes uridine-rich sequences. Distal adenine-binding and
lateral RNA-binding sites show considerable structural changes as compared to
bacterial Hfq. MjaHfq did not bind mononucleotides at these sites and would not
recognize single-stranded RNA as its bacterial homologues. Nevertheless, MjaHfq
possesses affinity to poly(A) RNA that seems to bind at the unstructured
positive-charged N-terminal tail of the protein.
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