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PDBsum entry 4v3c
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References listed in PDB file
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Key reference
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Title
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Complete switch from α-2,3- To α-2,6-Regioselectivity in pasteurella dagmatis β-D-Galactoside sialyltransferase by active-Site redesign.
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Authors
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K.Schmölzer,
T.Czabany,
C.Luley-Goedl,
T.Pavkov-Keller,
D.Ribitsch,
H.Schwab,
K.Gruber,
H.Weber,
B.Nidetzky.
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Ref.
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Chem Commun (camb), 2015,
51,
3083-3086.
[DOI no: ]
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PubMed id
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Abstract
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Structure-guided active-site redesign of a family GT-80 β-D-galactoside
sialyltransferase (from Pasteurella dagmatis) to change enzyme regioselectivity
from α-2,3 in the wild type to α-2,6 in a P7H-M117A double mutant is reported.
Biochemical data for sialylation of lactose together with protein crystal
structures demonstrate highly precise enzyme engineering.
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