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UniProt functional annotation for O34453 | ||
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| UniProt code: O34453. |
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| Organism: | |
Bacillus subtilis (strain 168). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus. |
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| Function: | |
Catalyzes the production of nitric oxide. {ECO:0000269|PubMed:11856757}. |
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| Catalytic activity: | |
Reaction=2 L-arginine + 4 O2 + 3 reduced [flavodoxin] = 5 H(+) + 4 H2O + 2 L-citrulline + 2 nitric oxide + 3 oxidized [flavodoxin]; Xref=Rhea:RHEA:52324, Rhea:RHEA-COMP:10622, Rhea:RHEA-COMP:10623, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16480, ChEBI:CHEBI:32682, ChEBI:CHEBI:57618, ChEBI:CHEBI:57743, ChEBI:CHEBI:58210; EC=1.14.14.47; Evidence={ECO:0000269|PubMed:11856757}; |
| Cofactor: | |
Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000269|PubMed:11856757}; |
| Cofactor: | |
Name=(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin; Xref=ChEBI:CHEBI:59560; Evidence={ECO:0000269|PubMed:11856757}; Note=Tetrahydrobiopterin (H4B). Can also use tetrahydrofolate (THF) in place of tetrahydrobiopterin (H4B) but binds THF with much lower affinity than H4B. {ECO:0000269|PubMed:11856757}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:11856757}. |
| Miscellaneous: | |
This protein is similar to the oxygenase domain of eukaryotic nitric oxide synthases but lacks the reductase domain which, in eukaryotes, is responsible for transfer of electrons to the ferric heme during nitric oxide synthesis. |
| Similarity: | |
Belongs to the NOS family. Bacterial NOS oxygenase subfamily. {ECO:0000305}. |
| Sequence caution: | |
Sequence=BAA22306.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.