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UniProt functional annotation for Q9F8A8 | ||
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| UniProt code: Q9F8A8. |
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| Organism: | |
Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901). |
| Taxonomy: | |
Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales; Thermoanaerobacteraceae; Carboxydothermus. |
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| Function: | |
CODH oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (possibly CooH). {ECO:0000269|PubMed:11489867}. |
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| Catalytic activity: | |
Reaction=CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:21040, Rhea:RHEA- COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:17245, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738; EC=1.2.7.4; Evidence={ECO:0000269|PubMed:11489867, ECO:0000269|PubMed:15113209, ECO:0000269|PubMed:18048691}; |
| Cofactor: | |
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000269|PubMed:11509720}; Note=Binds 3 [4Fe-4S] clusters per homodimer. {ECO:0000269|PubMed:11509720}; |
| Cofactor: | |
Name=[Ni-Fe-S] cluster; Xref=ChEBI:CHEBI:60400; Evidence={ECO:0000269|PubMed:11509720, ECO:0000269|PubMed:15113209, ECO:0000269|PubMed:18048691}; Note=Binds 2 [Ni-4Fe-5S] clusters per homodimer. {ECO:0000269|PubMed:11509720, ECO:0000269|PubMed:15113209, ECO:0000269|PubMed:18048691}; |
| Activity regulation: | |
Inactivated by O(2). |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:11509720}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:11489867}. Cell membrane {ECO:0000269|PubMed:11489867}; Peripheral membrane protein {ECO:0000269|PubMed:11489867}; Cytoplasmic side {ECO:0000269|PubMed:11489867}. Note=Loosely attached to the membrane, probably via CooF. |
| Domain: | |
Cluster B is an all-cysteinyl-liganded 4Fe-4S cluster; cluster C is a mixed Ni-Fe-S cluster which is the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe-4S cluster that bridges the two subunits of the CODH dimer. {ECO:0000269|PubMed:11509720, ECO:0000269|PubMed:15113209, ECO:0000269|PubMed:18048691}. |
| Similarity: | |
Belongs to the Ni-containing carbon monoxide dehydrogenase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.