PDBsum entry 4u3e

Oxidoreductase

PDB id: 4u3e

Contents

Protein chains

637 a.a.

Ligands

CIT ×2

GOL ×2

ACT

Metals

_ZN ×2

_NA

Waters ×894

References listed in PDB file

Key reference

• Title: The class III ribonucleotide reductase from neisseria bacilliformis can utilize thioredoxin as a reductant.
• Authors: Y.Wei, M.A.Funk, L.A.Rosado, J.Baek, C.L.Drennan, J.Stubbe.
• Ref.: Proc Natl Acad Sci U S A, 2014, 111, E3756. [DOI no: 10.1073/pnas.1414396111]
• PubMed id: 25157154

Abstract

The class III anaerobic ribonucleotide reductases (RNRs) studied to date couple the reduction of ribonucleotides to deoxynucleotides with the oxidation of formate to CO2. Here we report the cloning and heterologous expression of the Neisseria bacilliformis class III RNR and show that it can catalyze nucleotide reduction using the ubiquitous thioredoxin/thioredoxin reductase/NADPH system. We present a structural model based on a crystal structure of the homologous Thermotoga maritima class III RNR, showing its architecture and the position of conserved residues in the active site. Phylogenetic studies suggest that this form of class III RNR is present in bacteria and archaea that carry out diverse types of anaerobic metabolism.