UniProt functional annotation for Q03555



	UniProt functional annotation for Q03555

UniProt code: Q03555.

Organism: Rattus norvegicus (Rat).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.

Function: Microtubule-associated protein involved in membrane protein- cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (PubMed:8264797). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (By similarity). {ECO:0000250|UniProtKB:Q8BUV3, ECO:0000269|PubMed:8264797}.

Function: Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. {ECO:0000269|PubMed:9990024}.

Catalytic activity: Reaction=ATP + H(+) + molybdopterin = adenylyl-molybdopterin + diphosphate; Xref=Rhea:RHEA:31331, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58698, ChEBI:CHEBI:62727; EC=2.7.7.75; Evidence={ECO:0000305|PubMed:9990024}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31332; Evidence={ECO:0000305|PubMed:9990024};

Catalytic activity: Reaction=adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo- molybdopterin; Xref=Rhea:RHEA:35047, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:36264, ChEBI:CHEBI:62727, ChEBI:CHEBI:71302, ChEBI:CHEBI:456215; EC=2.10.1.1; Evidence={ECO:0000305|PubMed:9990024}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35048; Evidence={ECO:0000305|PubMed:9990024};

Cofactor: Name=Mg(2+); Xref=ChEBI:CHEBI:18420;

Activity regulation: Inhibited by copper and tungsten. {ECO:0000250}.

Pathway: Cofactor biosynthesis; molybdopterin biosynthesis. {ECO:0000305|PubMed:9990024}.

Subunit: Homotrimer, homodimer and homooligomer (PubMed:11325967, PubMed:25137389, PubMed:25531214). Interacts with SRGAP2 (via SH3 domain) (By similarity). Interacts with GLRB (PubMed:15201864, PubMed:16511563, PubMed:25137389, PubMed:25531214). Interacts with GABARAP (PubMed:10900017). Interacts with GABRA3 (PubMed:25531214). GABRA3 and GLRB occupy overlapping binding sites (PubMed:25531214). Interacts with ARHGAP32; IQSEC3, INSYN1 and INSYN2A (By similarity). {ECO:0000250|UniProtKB:Q8BUV3, ECO:0000269|PubMed:10900017, ECO:0000269|PubMed:11325967, ECO:0000269|PubMed:15201864, ECO:0000269|PubMed:16511563}.

Subcellular location: Cell junction, synapse, postsynaptic cell membrane {ECO:0000269|PubMed:16511563}; Lipid-anchor {ECO:0000305|PubMed:16511563}; Cytoplasmic side {ECO:0000269|PubMed:16511563}. Cell membrane {ECO:0000269|PubMed:12684523}; Lipid-anchor {ECO:0000305|PubMed:12684523}; Cytoplasmic side {ECO:0000305|PubMed:12684523}. Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9NQX3}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:16511563}. Cell projection, dendrite {ECO:0000250|UniProtKB:Q9NQX3}. Cell junction, synapse, postsynaptic density {ECO:0000250|UniProtKB:Q8BUV3}. Note=Cytoplasmic face of glycinergic postsynaptic membranes (PubMed:16511563). Forms clusters at synapses (By similarity). {ECO:0000250|UniProtKB:Q8BUV3, ECO:0000269|PubMed:16511563}.

Tissue specificity: Expressed in tissues including spinal cord, brain, liver, kidney and lung.

Ptm: Phosphorylated.

Ptm: Palmitoylated. Palmitoylation is stimulated by GABA type A receptors activity. Palmitoylation by ZDHHC12 regulates clustering at synapses. {ECO:0000250|UniProtKB:Q8BUV3}.

Similarity: In the N-terminal section; belongs to the MoaB/Mog family. {ECO:0000305}.

Similarity: In the C-terminal section; belongs to the MoeA family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Rattus norvegicus (Rat).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.



Function:		Microtubule-associated protein involved in membrane protein- cytoskeleton interactions. It is thought to anchor the inhibitory glycine receptor (GLYR) to subsynaptic microtubules (PubMed:8264797). Acts as a major instructive molecule at inhibitory synapses, where it also clusters GABA type A receptors (By similarity). {ECO:0000250\|UniProtKB:Q8BUV3, ECO:0000269\|PubMed:8264797}.



Function:		Has also a catalytic activity and catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. {ECO:0000269\|PubMed:9990024}.


Catalytic activity:		Reaction=ATP + H(+) + molybdopterin = adenylyl-molybdopterin + diphosphate; Xref=Rhea:RHEA:31331, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58698, ChEBI:CHEBI:62727; EC=2.7.7.75; Evidence={ECO:0000305\|PubMed:9990024}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:31332; Evidence={ECO:0000305\|PubMed:9990024};
Catalytic activity:		Reaction=adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo- molybdopterin; Xref=Rhea:RHEA:35047, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:36264, ChEBI:CHEBI:62727, ChEBI:CHEBI:71302, ChEBI:CHEBI:456215; EC=2.10.1.1; Evidence={ECO:0000305\|PubMed:9990024}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35048; Evidence={ECO:0000305\|PubMed:9990024};
Cofactor:		Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Activity regulation:		Inhibited by copper and tungsten. {ECO:0000250}.
Pathway:		Cofactor biosynthesis; molybdopterin biosynthesis. {ECO:0000305\|PubMed:9990024}.
Subunit:		Homotrimer, homodimer and homooligomer (PubMed:11325967, PubMed:25137389, PubMed:25531214). Interacts with SRGAP2 (via SH3 domain) (By similarity). Interacts with GLRB (PubMed:15201864, PubMed:16511563, PubMed:25137389, PubMed:25531214). Interacts with GABARAP (PubMed:10900017). Interacts with GABRA3 (PubMed:25531214). GABRA3 and GLRB occupy overlapping binding sites (PubMed:25531214). Interacts with ARHGAP32; IQSEC3, INSYN1 and INSYN2A (By similarity). {ECO:0000250\|UniProtKB:Q8BUV3, ECO:0000269\|PubMed:10900017, ECO:0000269\|PubMed:11325967, ECO:0000269\|PubMed:15201864, ECO:0000269\|PubMed:16511563}.
Subcellular location:		Cell junction, synapse, postsynaptic cell membrane {ECO:0000269\|PubMed:16511563}; Lipid-anchor {ECO:0000305\|PubMed:16511563}; Cytoplasmic side {ECO:0000269\|PubMed:16511563}. Cell membrane {ECO:0000269\|PubMed:12684523}; Lipid-anchor {ECO:0000305\|PubMed:12684523}; Cytoplasmic side {ECO:0000305\|PubMed:12684523}. Cytoplasm, cytosol {ECO:0000250\|UniProtKB:Q9NQX3}. Cytoplasm, cytoskeleton {ECO:0000269\|PubMed:16511563}. Cell projection, dendrite {ECO:0000250\|UniProtKB:Q9NQX3}. Cell junction, synapse, postsynaptic density {ECO:0000250\|UniProtKB:Q8BUV3}. Note=Cytoplasmic face of glycinergic postsynaptic membranes (PubMed:16511563). Forms clusters at synapses (By similarity). {ECO:0000250\|UniProtKB:Q8BUV3, ECO:0000269\|PubMed:16511563}.
Tissue specificity:		Expressed in tissues including spinal cord, brain, liver, kidney and lung.
Ptm:		Phosphorylated.
Ptm:		Palmitoylated. Palmitoylation is stimulated by GABA type A receptors activity. Palmitoylation by ZDHHC12 regulates clustering at synapses. {ECO:0000250\|UniProtKB:Q8BUV3}.
Similarity:		In the N-terminal section; belongs to the MoaB/Mog family. {ECO:0000305}.
Similarity:		In the C-terminal section; belongs to the MoeA family. {ECO:0000305}.