PDBsum entry 4rkb

Hydrolase

PDB id: 4rkb

Contents

Protein chain

129 a.a.

Ligands

THP

Metals

_CA

Waters ×65

PDB id:

4rkb

Name:

Hydrolase

Title:

Crystal structure of staphylococcal nuclease variant delta+phs v66t/v99t at cryogenic temperature

Structure:

Thermonuclease. Chain: a. Fragment: unp residues 83-232. Engineered: yes

Source:

Staphylococcus aureus. Organism_taxid: 1280. Gene: nuc. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.88Å R-factor: 0.187 R-free: 0.225

Authors:

J.A.Caro,J.L.Schlessman,B.Garcia-Moreno E.

Key ref:

J.A.Caro et al. Cavities in proteins. To be published, .

Date:

12-Oct-14 Release date: 26-Nov-14

Protein chain

P00644  (NUC_STAAU) -  Thermonuclease from Staphylococcus aureus

Seq: 231 a.a.

Struc: 129 a.a.*

* PDB and UniProt seqs differ at 7 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.1.31.1 - micrococcal nuclease.
• Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.