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UniProt functional annotation for Q81MI9 | ||
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| UniProt code: Q81MI9. |
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| Organism: | |
Bacillus anthracis. |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus cereus group. |
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| Function: | |
Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway. {ECO:0000255|HAMAP-Rule:MF_01682}. |
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| Catalytic activity: | |
Reaction=1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3- (methylsulfanyl)propanoate + CO + formate + 2 H(+); Xref=Rhea:RHEA:14161, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:17245, ChEBI:CHEBI:49016, ChEBI:CHEBI:49252; EC=1.13.11.53; Evidence={ECO:0000255|HAMAP- Rule:MF_01682}; |
| Catalytic activity: | |
Reaction=1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4- methylsulfanyl-2-oxobutanoate + formate + 2 H(+); Xref=Rhea:RHEA:24504, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:16723, ChEBI:CHEBI:49252; EC=1.13.11.54; Evidence={ECO:0000255|HAMAP-Rule:MF_01682}; |
| Cofactor: | |
Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence={ECO:0000255|HAMAP-Rule:MF_01682}; Note=Binds 1 Fe cation per monomer. {ECO:0000255|HAMAP-Rule:MF_01682}; |
| Cofactor: | |
Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000255|HAMAP-Rule:MF_01682}; Note=Binds 1 nickel ion per monomer. {ECO:0000255|HAMAP-Rule:MF_01682}; |
| Pathway: | |
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6. {ECO:0000255|HAMAP-Rule:MF_01682}. |
| Subunit: | |
Monomer. {ECO:0000255|HAMAP-Rule:MF_01682}. |
| Similarity: | |
Belongs to the acireductone dioxygenase (ARD) family. {ECO:0000255|HAMAP-Rule:MF_01682}. |
Annotations taken from UniProtKB at the EBI.