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PDBsum entry 4pqq
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Protein binding
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PDB id
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4pqq
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References listed in PDB file
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Key reference
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Title
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Structure of mouse muskelin discoidin domain and biochemical characterization of its self-Association.
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Authors
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K.H.Kim,
S.K.Hong,
K.Y.Hwang,
E.E.Kim.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2014,
70,
2863-2874.
[DOI no: ]
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PubMed id
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Abstract
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Muskelin is an intracellular kelch-repeat protein comprised of discoidin, LisH,
CTLH and kelch-repeat domains. It is involved in cell adhesion and the
regulation of cytoskeleton dynamics as well as being a component of a putative
E3 ligase complex. Here, the first crystal structure of mouse muskelin discoidin
domain (MK-DD) is reported at 1.55 Å resolution, which reveals a distorted
eight-stranded β-barrel with two short α-helices at one end of the barrel.
Interestingly, the N- and C-termini are not linked by the disulfide bonds found
in other eukaryotic discoidin structures. A highly conserved MIND motif appears
to be the determinant for MK-DD specific interaction together with the spike
loops. Analysis of interdomain interaction shows that MK-DD binds the
kelch-repeat domain directly and that this interaction depends on the presence
of the LisH domain.
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