UniProt functional annotation for Q04609



	UniProt functional annotation for Q04609

UniProt code: Q04609.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.

Function: Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.

Catalytic activity: Reaction=Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.; EC=3.4.17.21;

Cofactor: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:16467855, ECO:0000269|PubMed:17372356, ECO:0000269|PubMed:17567119, ECO:0000269|PubMed:18234225, ECO:0000269|PubMed:19053759, ECO:0000269|PubMed:19301871}; Note=Binds 2 Zn(2+) ions per subunit. Required for NAALADase activity. {ECO:0000269|PubMed:16467855};

Activity regulation: The NAALADase activity is inhibited by beta-NAAG, quisqualic acid, 2-(phosphonomethyl) pentanedioic acid (PMPA) and EDTA. Activated by cobalt. {ECO:0000269|PubMed:17567119}.

Biophysicochemical properties: pH dependence: Stable at pH greater than 6.5.;

Subunit: Homodimer. {ECO:0000269|PubMed:16467855, ECO:0000269|PubMed:19301871}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:9809977}; Single-pass type II membrane protein {ECO:0000269|PubMed:9809977}.

Subcellular location: [Isoform PSMA']: Cytoplasm {ECO:0000269|PubMed:9809977}.

Tissue specificity: Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-3 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer. {ECO:0000269|PubMed:11092759, ECO:0000269|PubMed:14716746, ECO:0000269|PubMed:16555021, ECO:0000269|PubMed:17150306, ECO:0000269|PubMed:9375657}.

Induction: In the prostate, up-regulated in response to androgen deprivation.

Domain: The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal. {ECO:0000269|PubMed:9187245}.

Ptm: The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.

Ptm: The N-terminus is blocked.

Polymorphism: Genetic variation in FOLH1 may be associated with low folate levels and consequent hyperhomocysteinemia. This condition can result in increased risk of cardiovascular disease, neural tube defects, and cognitive deficits.

Miscellaneous: PSMA is used as a diagnostic and prognostic indicator of prostate cancer, and as a possible marker for various neurological disorders such as schizophrenia, Alzheimer disease and Huntington disease.

Miscellaneous: [Isoform PSMA-3]: Incomplete sequence. {ECO:0000305}.

Miscellaneous: [Isoform PSMA-4]: Incomplete sequence. {ECO:0000305}.

Similarity: Belongs to the peptidase M28 family. M28B subfamily. {ECO:0000305}.

Sequence caution: Sequence=AAF31167.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.



Function:		Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.


Catalytic activity:		Reaction=Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.; EC=3.4.17.21;
Cofactor:		Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269\|PubMed:16467855, ECO:0000269\|PubMed:17372356, ECO:0000269\|PubMed:17567119, ECO:0000269\|PubMed:18234225, ECO:0000269\|PubMed:19053759, ECO:0000269\|PubMed:19301871}; Note=Binds 2 Zn(2+) ions per subunit. Required for NAALADase activity. {ECO:0000269\|PubMed:16467855};
Activity regulation:		The NAALADase activity is inhibited by beta-NAAG, quisqualic acid, 2-(phosphonomethyl) pentanedioic acid (PMPA) and EDTA. Activated by cobalt. {ECO:0000269\|PubMed:17567119}.
Biophysicochemical properties:		pH dependence: Stable at pH greater than 6.5.;
Subunit:		Homodimer. {ECO:0000269\|PubMed:16467855, ECO:0000269\|PubMed:19301871}.
Subcellular location:		Cell membrane {ECO:0000269\|PubMed:9809977}; Single-pass type II membrane protein {ECO:0000269\|PubMed:9809977}.
Subcellular location:		[Isoform PSMA']: Cytoplasm {ECO:0000269\|PubMed:9809977}.
Tissue specificity:		Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-3 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer. {ECO:0000269\|PubMed:11092759, ECO:0000269\|PubMed:14716746, ECO:0000269\|PubMed:16555021, ECO:0000269\|PubMed:17150306, ECO:0000269\|PubMed:9375657}.
Induction:		In the prostate, up-regulated in response to androgen deprivation.
Domain:		The NAALADase activity is found in the central region, the dipeptidyl peptidase IV type activity in the C-terminal. {ECO:0000269\|PubMed:9187245}.
Ptm:		The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
Ptm:		The N-terminus is blocked.
Polymorphism:		Genetic variation in FOLH1 may be associated with low folate levels and consequent hyperhomocysteinemia. This condition can result in increased risk of cardiovascular disease, neural tube defects, and cognitive deficits.
Miscellaneous:		PSMA is used as a diagnostic and prognostic indicator of prostate cancer, and as a possible marker for various neurological disorders such as schizophrenia, Alzheimer disease and Huntington disease.
Miscellaneous:		[Isoform PSMA-3]: Incomplete sequence. {ECO:0000305}.
Miscellaneous:		[Isoform PSMA-4]: Incomplete sequence. {ECO:0000305}.
Similarity:		Belongs to the peptidase M28 family. M28B subfamily. {ECO:0000305}.
Sequence caution:		Sequence=AAF31167.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};