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UniProt functional annotation for J3F2B0 | ||
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| UniProt code: J3F2B0. |
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| Organism: | |
Actinomyces naeslundii (strain ATCC 12104 / DSM 43013 / CCUG 2238 / JCM 8349 / NCTC 10301 / Howell 279). |
| Taxonomy: | |
Bacteria; Actinobacteria; Actinomycetales; Actinomycetaceae; Actinomyces. |
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| Function: | |
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans- encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity (By similarity). {ECO:0000250, ECO:0000269|PubMed:24505130}. |
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| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:24505130}; Note=Binds 2 Mg(2+) per subunit. {ECO:0000269|PubMed:24505130}; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:24505130}; Note=Binds 2 Mn(2+) per subunit. {ECO:0000269|PubMed:24505130}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:24505130}; Note=Binds 1 Zn(2+) per subunit, which may stabilize the HNH Cas9 architecture. {ECO:0000269|PubMed:24505130}; |
| Subunit: | |
Monomer. Binds crRNA and tracrRNA (Probable). {ECO:0000305|PubMed:24505130}. |
| Domain: | |
Has 2 endonuclease domains. The discontinuous RuvC-like domain cleaves the target DNA noncomplementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA (Probable). {ECO:0000305|PubMed:24505130}. |
| Biotechnology: | |
The simplicity of the Cas9-gRNAs RNA-directed DNA endonuclease activity may be used to target and modify a DNA sequence of interest. |
| Similarity: | |
Belongs to the CRISPR-associated protein Cas9 family. Subtype II-C subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.