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UniProt functional annotation for Q8I295 | ||
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| UniProt code: Q8I295. |
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| Organism: | |
Plasmodium falciparum (isolate 3D7). |
| Taxonomy: | |
Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodiidae; Plasmodium; Plasmodium (Laverania). |
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| Function: | |
Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis. {ECO:0000269|PubMed:16289098}. |
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| Catalytic activity: | |
Reaction=dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]- [ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:24825, Rhea:RHEA- COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57623, ChEBI:CHEBI:128753; EC=1.17.7.4; Evidence={ECO:0000269|PubMed:16289098}; |
| Catalytic activity: | |
Reaction=H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]- [ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:24488, Rhea:RHEA- COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:128753, ChEBI:CHEBI:128769; EC=1.17.7.4; Evidence={ECO:0000269|PubMed:16289098}; |
| Cofactor: | |
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000269|PubMed:24188825}; Note=Was shown to bind 1 [3Fe-4S] cluster (PubMed:24188825). However, it initially contains a [4Fe-4S] cluster which easily degrades into a [3Fe-4S] form in the presence of oxygen (Probable). {ECO:0000269|PubMed:24188825, ECO:0000305|PubMed:24188825}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=39 uM for (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) (with methyl viologen as electron donor and at 30 degrees Celsius) {ECO:0000269|PubMed:16289098}; Vmax=2.1 umol/min/mg enzyme for (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) (with methyl viologen as electron donor and at 30 degrees Celsius) {ECO:0000269|PubMed:16289098}; Note=kcat is 1.3 sec(-1) for (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) (with methyl viologen as electron donor and at 30 degrees Celsius). {ECO:0000269|PubMed:16289098}; Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:16289098}; |
| Pathway: | |
Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3- methylbutenyl diphosphate: step 1/1. {ECO:0000269|PubMed:16289098}. |
| Pathway: | |
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 6/6. {ECO:0000269|PubMed:16289098}. |
| Subunit: | |
Interacts with Fd/ferredoxin. {ECO:0000269|PubMed:16289098}. |
| Subcellular location: | |
Plastid, apicoplast {ECO:0000305}. |
| Similarity: | |
Belongs to the IspH family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.