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UniProt functional annotation for P37355 | ||
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| UniProt code: P37355. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes a proton abstraction reaction that results in 2,5- elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6- hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro. {ECO:0000269|PubMed:15808744, ECO:0000269|PubMed:18284213}. |
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| Catalytic activity: | |
Reaction=5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1- carboxylate = (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1- carboxylate + pyruvate; Xref=Rhea:RHEA:25597, ChEBI:CHEBI:15361, ChEBI:CHEBI:58689, ChEBI:CHEBI:58818; EC=4.2.99.20; Evidence={ECO:0000255|HAMAP-Rule:MF_01660, ECO:0000269|PubMed:18284213}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=10.1 uM for SEPHCHC (at 25 degrees Celsius and pH 7) {ECO:0000269|PubMed:18284213}; |
| Pathway: | |
Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 3/7. {ECO:0000255|HAMAP-Rule:MF_01660}. |
| Pathway: | |
Quinol/quinone metabolism; menaquinone biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01660}. |
| Subunit: | |
Monomer. {ECO:0000255|HAMAP-Rule:MF_01660, ECO:0000269|PubMed:18284213}. |
| Similarity: | |
Belongs to the AB hydrolase superfamily. MenH family. {ECO:0000255|HAMAP-Rule:MF_01660, ECO:0000305}. |
| Sequence caution: | |
Sequence=AAA24151.1; Type=Frameshift; Evidence={ECO:0000305}; Sequence=M93421; Type=Frameshift; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.