UniProt functional annotation for Q47149



	UniProt functional annotation for Q47149

UniProt code: Q47149.

Organism: Escherichia coli (strain K12).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.

Function: Toxic component of a type II toxin-antitoxin (TA) system (PubMed:17263853). A sequence-specific mRNA endoribonuclease that inhibits translation elongation and induces bacterial stasis (PubMed:19210620). Cleavage occurs between the second and third residue of the Lys codon followed by a G or A (5'AAA(G/A)3'), is reading-frame dependent and occurs within the 5' end of most mRNAs (PubMed:19210620). Ribosome-binding confers the sequence specificity and reading frame- dependence (PubMed:19210620). When overexpressed in liquid media YafQ partially inhibits protein synthesis, with a reduction in growth rate and colony growth rate. This effect is counteracted by coexpression with cognate antitoxin DinJ (PubMed:17263853). YafQ and DinJ together bind their own promoter, and repress its expression (PubMed:24898247). {ECO:0000269|PubMed:17263853, ECO:0000269|PubMed:19210620, ECO:0000269|PubMed:24898247}.

Function: Cell death governed by the MazE-MazF and DinJ-YafQ TA systems seems to play a role in biofilm formation (PubMed:19707553). {ECO:0000269|PubMed:19707553}.

Subunit: Monomer in the absence of antitoxin (PubMed:24898247). Forms a heterotetramer with antitoxin DinJ, with 2 YafQ-DinJ dimers associated via the N-terminus of the DinJ antitoxins (YafQ-(DinJ)2-YafQ) (PubMed:17263853, PubMed:24898247). In this complex the toxin activity is inhibited. Binds the 70S ribosome via the 50S ribosomal subunit (PubMed:19210620). {ECO:0000269|PubMed:17263853, ECO:0000269|PubMed:19210620, ECO:0000269|PubMed:24898247}.

Developmental stage: May function as a drug tolerance determinant in biofilm, but not stationary phase planktonic cells. {ECO:0000269|PubMed:19707553}.

Induction: By the DNA damaging agent mitomycin C. {ECO:0000269|PubMed:19210620}.

Disruption phenotype: Cells missing yafQ show increased biofilm sensitivity to the antibiotics cefazolin (a beta-lactam inhibitor) and tobramycin (a protein synthesis inhibitor). There is no difference in antibiotic sensitivity in stationary phase planktonic cells (PubMed:19307375). {ECO:0000269|PubMed:19307375, ECO:0000269|PubMed:19707553}.

Similarity: Belongs to the RelE toxin family. YafQ subfamily. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Escherichia coli (strain K12).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.



Function:		Toxic component of a type II toxin-antitoxin (TA) system (PubMed:17263853). A sequence-specific mRNA endoribonuclease that inhibits translation elongation and induces bacterial stasis (PubMed:19210620). Cleavage occurs between the second and third residue of the Lys codon followed by a G or A (5'AAA(G/A)3'), is reading-frame dependent and occurs within the 5' end of most mRNAs (PubMed:19210620). Ribosome-binding confers the sequence specificity and reading frame- dependence (PubMed:19210620). When overexpressed in liquid media YafQ partially inhibits protein synthesis, with a reduction in growth rate and colony growth rate. This effect is counteracted by coexpression with cognate antitoxin DinJ (PubMed:17263853). YafQ and DinJ together bind their own promoter, and repress its expression (PubMed:24898247). {ECO:0000269\|PubMed:17263853, ECO:0000269\|PubMed:19210620, ECO:0000269\|PubMed:24898247}.



Function:		Cell death governed by the MazE-MazF and DinJ-YafQ TA systems seems to play a role in biofilm formation (PubMed:19707553). {ECO:0000269\|PubMed:19707553}.


Subunit:		Monomer in the absence of antitoxin (PubMed:24898247). Forms a heterotetramer with antitoxin DinJ, with 2 YafQ-DinJ dimers associated via the N-terminus of the DinJ antitoxins (YafQ-(DinJ)2-YafQ) (PubMed:17263853, PubMed:24898247). In this complex the toxin activity is inhibited. Binds the 70S ribosome via the 50S ribosomal subunit (PubMed:19210620). {ECO:0000269\|PubMed:17263853, ECO:0000269\|PubMed:19210620, ECO:0000269\|PubMed:24898247}.
Developmental stage:		May function as a drug tolerance determinant in biofilm, but not stationary phase planktonic cells. {ECO:0000269\|PubMed:19707553}.
Induction:		By the DNA damaging agent mitomycin C. {ECO:0000269\|PubMed:19210620}.
Disruption phenotype:		Cells missing yafQ show increased biofilm sensitivity to the antibiotics cefazolin (a beta-lactam inhibitor) and tobramycin (a protein synthesis inhibitor). There is no difference in antibiotic sensitivity in stationary phase planktonic cells (PubMed:19307375). {ECO:0000269\|PubMed:19307375, ECO:0000269\|PubMed:19707553}.
Similarity:		Belongs to the RelE toxin family. YafQ subfamily. {ECO:0000305}.