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PDBsum entry 4mb3
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References listed in PDB file
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Key reference
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Title
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Crystal structures of substrate-Bound chitinase from the psychrophilic bacterium moritella marina and its structure in solution.
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Authors
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P.H.Malecki,
C.E.Vorgias,
M.V.Petoukhov,
D.I.Svergun,
W.Rypniewski.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2014,
70,
676-684.
[DOI no: ]
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PubMed id
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Abstract
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The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is
outstanding in its complexity. Many glycoside hydrolases, such as chitinases and
cellulases, have multi-domain structures, but only a few have been solved. The
flexibility of the hinge regions between the domains apparently makes these
proteins difficult to crystallize. The analysis of an active-site mutant of
MmChi60 in an unliganded form and in complex with the substrates NAG4 and NAG5
revealed significant differences in the substrate-binding site compared with the
previously determined complexes of most studied chitinases. A SAXS experiment
demonstrated that in addition to the elongated state found in the crystal, the
protein can adapt other conformations in solution ranging from fully extended to
compact.
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Secondary reference #1
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Title
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Structure of a complete four-Domain chitinase from moritella marina, A marine psychrophilic bacterium.
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Authors
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P.H.Malecki,
J.E.Raczynska,
C.E.Vorgias,
W.Rypniewski.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2013,
69,
821-829.
[DOI no: ]
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PubMed id
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