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UniProt functional annotation for Q9K499 | ||
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| UniProt code: Q9K499. |
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| Organism: | |
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145). |
| Taxonomy: | |
Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces albidoflavus group. |
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| Function: | |
Catalyzes the cyclization of farnesyl diphosphate (FPP) to the sesquiterpene epi-isozizaene. {ECO:0000269|PubMed:16669656}. |
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| Catalytic activity: | |
Reaction=(2E,6E)-farnesyl diphosphate = (+)-epi-isozizaene + diphosphate; Xref=Rhea:RHEA:25992, ChEBI:CHEBI:33019, ChEBI:CHEBI:51458, ChEBI:CHEBI:175763; EC=4.2.3.37; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:16669656}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:16669656}; Name=Fe(3+); Xref=ChEBI:CHEBI:29034; Evidence={ECO:0000269|PubMed:16669656}; Note=Binds 3 Mg(2+) ions per subunit. Can also use Mn(2+) and Fe(3+) but less efficiently, and other divalent cations such as Zn(2+), Fe(2+), Co(2+), Cu(2+) and Ni(2+) are nearly completely inefficient as cofactors. {ECO:0000269|PubMed:16669656}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=147 nM for FPP {ECO:0000269|PubMed:16669656}; |
| Pathway: | |
Sesquiterpene biosynthesis; epi-isozizaene biosynthesis. |
| Domain: | |
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+). |
| Similarity: | |
Belongs to the terpene synthase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.