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PDBsum entry 4lx5
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References listed in PDB file
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Key reference
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Title
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Modular riboswitch toolsets for synthetic genetic control in diverse bacterial species.
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Authors
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C.J.Robinson,
H.A.Vincent,
M.C.Wu,
P.T.Lowe,
M.S.Dunstan,
D.Leys,
J.Micklefield.
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Ref.
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J Am Chem Soc, 2014,
136,
10615-10624.
[DOI no: ]
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PubMed id
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Abstract
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Ligand-dependent control of gene expression is essential for gene functional
analysis, target validation, protein production, and metabolic engineering.
However, the expression tools currently available are difficult to transfer
between species and exhibit limited mechanistic diversity. Here we demonstrate
how the modular architecture of purine riboswitches can be exploited to develop
orthogonal and chimeric switches that are transferable across diverse bacterial
species, modulating either transcription or translation, to provide tunable
activation or repression of target gene expression, in response to synthetic
non-natural effector molecules. Our novel riboswitch-ligand pairings are shown
to regulate physiologically important genes required for bacterial motility in
Escherichia coli and cell morphology in Bacillus subtilis. These findings are
relevant for future gene function studies and antimicrobial target validation,
while providing new modular and orthogonal regulatory components for deployment
in synthetic biology regimes.
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Headers
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